响应急性热应激时功能相关基因集的动态解析
Dynamic resolution of functionally related gene sets in response to acute heat stress.
作者信息
Szustakowski Joseph D, Kosinski Penelope A, Marrese Christine A, Lee Jee-Hyung, Elliman Stephen J, Nirmala Nanguneri, Kemp Daniel M
机构信息
Developmental and Molecular Pathways, Novartis Institutes for BioMedical Research, Cambridge, Massachusetts, USA.
出版信息
BMC Mol Biol. 2007 Jun 5;8:46. doi: 10.1186/1471-2199-8-46.
BACKGROUND
Using a gene clustering strategy we determined intracellular pathway relationships within skeletal myotubes in response to an acute heat stress stimuli. Following heat shock, the transcriptome was analyzed by microarray in a temporal fashion to characterize the dynamic relationship of signaling pathways.
RESULTS
Bioinformatics analyses exposed coordination of functionally-related gene sets, depicting mechanism-based responses to heat shock. Protein turnover-related pathways were significantly affected including protein folding, pre-mRNA processing, mRNA splicing, proteolysis and proteasome-related pathways. Many responses were transient, tending to normalize within 24 hours.
CONCLUSION
In summary, we show that the transcriptional response to acute cell stress is largely transient and proteosome-centric.
背景
我们采用基因聚类策略确定了骨骼肌肌管内对急性热应激刺激的细胞内信号通路关系。热休克后，通过微阵列以时间序列方式分析转录组，以表征信号通路的动态关系。
结果
生物信息学分析揭示了功能相关基因集的协调性，描绘了基于机制的热休克反应。与蛋白质周转相关的通路受到显著影响，包括蛋白质折叠、前体mRNA加工、mRNA剪接、蛋白水解和蛋白酶体相关通路。许多反应是短暂的，倾向于在24小时内恢复正常。
结论
总之，我们表明对急性细胞应激的转录反应在很大程度上是短暂的且以蛋白酶体为中心。
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