酵母苯丙氨酸tRNA反密码子臂的核糖和脱氧核糖类似物与大肠杆菌和兔肝的程序化小核糖体亚基的相互作用。
Interaction of ribo- and deoxyriboanalogs of yeast tRNA(Phe) anticodon arm with programmed small ribosomal subunits of Escherichia coli and rabbit liver.
作者信息
Koval'chuke O V, Potapov A P, El'skaya A V, Potapov V K, Krinetskaya N F, Dolinnaya N G, Shabarova Z A
机构信息
Institute of Molecular Biology and Genetics, Kiev, USSR.
出版信息
Nucleic Acids Res. 1991 Aug 11;19(15):4199-201. doi: 10.1093/nar/19.15.4199.
A synthetic ribooligonucleotide, r(CCAGACUGm-AAGAUCUGG), corresponding to the unmodified yeast tRNA(Phe) anticodon arm is shown to bind to poly(U) programmed small ribosomal subunits of both E. coli and rabbit liver with affinity two order less than that of a natural anticodon arm. Its deoxyriboanalogs d(CCAGACTGAAGATCTGG) and d(CCAGA)r(CUGm-AAGA)d(TCTGG), are used to study the influence of sugar-phosphate modification on the interaction of tRNA with programmed small ribosomal subunits. The deoxyribooligonucleotide is shown to adopt a hairpin structure. Nevertheless, as well as oligonucleotide with deoxyriboses in stem region, it is not able to bind to 30S or 40S ribosomal subunits in the presence of ribo-(poly(U] or deoxyribo-(poly (dT) template. The deoxyribooligonucleotide also has no inhibitory effect on tRNA(Phe) binding to 30S ribosomes at 10-fold excess over tRNA. Neomycin does not influence binding of tRNA anticodon arm analogs used. Complete tRNA molecule and natural modifications of anticodon arm are considered to stabilize the arm structure needed for its interaction with a programmed ribosome.
一种与未修饰的酵母tRNA（Phe）反密码子臂相对应的合成核糖寡核苷酸r（CCAGACUGm - AAGAUCUGG），被证明能与大肠杆菌和兔肝脏的聚（U）编程小核糖体亚基结合，但其亲和力比天然反密码子臂低两个数量级。它的脱氧核糖类似物d（CCAGACTGAAGATCTGG）和d（CCAGA）r（CUGm - AAGA）d（TCTGG），用于研究糖 - 磷酸修饰对tRNA与编程小核糖体亚基相互作用的影响。该脱氧核糖寡核苷酸显示出呈发夹结构。然而，与茎区含有脱氧核糖的寡核苷酸一样，在存在核糖 - （聚（U））或脱氧核糖 - （聚（dT））模板的情况下，它无法与30S或40S核糖体亚基结合。该脱氧核糖寡核苷酸在比tRNA过量10倍时，对tRNA（Phe）与30S核糖体的结合也没有抑制作用。新霉素不影响所使用的tRNA反密码子臂类似物的结合。完整的tRNA分子和反密码子臂的天然修饰被认为能稳定其与编程核糖体相互作用所需的臂结构。
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