与核糖体聚尿苷酸编程的P位点接触的转运RNA区域。
tRNA regions which contact with the ribosomal poly(U)-programmed P-site.
作者信息
Nekhai S A, Parfenov D V, Saminsky E M
机构信息
Division of Radiation and Molecular Biophysics, Petersburg Nuclear Physics Institute, Russian Academy of Sciences, Gatchina, Leningrad region.
出版信息
Biochim Biophys Acta. 1994 Aug 2;1218(3):481-4. doi: 10.1016/0167-4781(94)90212-7.
Equilibrium binding affinity of yeast tRNA(Phe) for Escherichia coli poly(U)-programmed 70S ribosomal P-site was compared with corresponding affinities of several tRNA(Phe) 3'- and 5'-end-truncated derivatives, all containing the anticodon arm. Our findings strongly suggest that besides three 3'-terminal-CCA nucleotides (C74, C75 and A76), only the tRNA(Phe) anticodon arm (N28-N42) contains ribosomal P-site contact centers and that there are no such centers in the intermediate regions N1-N27 and N43-N73.
将酵母苯丙氨酸转运核糖核酸（tRNA(Phe)）与大肠杆菌聚尿嘧啶（poly(U)）编程的70S核糖体P位点的平衡结合亲和力，与几种均含有反密码子臂的3'端和5'端截短的tRNA(Phe)衍生物的相应亲和力进行了比较。我们的研究结果有力地表明，除了三个3'端的CCA核苷酸（C74、C75和A76）外，只有tRNA(Phe)的反密码子臂（N28 - N42）含有核糖体P位点接触中心，而在中间区域N1 - N27和N43 - N73中不存在这样的中心。
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