Han Xu, Wong Yuk Shan, Wong Ming Hung, Tam Nora Fung Yee
Department of Biology and Chemistry, City University of Hong Kong, Kowloon, Hong Kong SAR, China.
Water Environ Res. 2008 Jul;80(7):647-53.
The feasibility of obtaining and using the biomass of a microalga, Chlorella miniata, from domestic wastewater (DW) cultures for the removal of chromium(III) [Cr(III)] and chromium(VI) [Cr(VI)] was compared with that from commercial Bristol medium (BM). Results showed that Chlorella miniata cultured in DW under 16-8 hours light-dark cycle [DW(16-8)] had similar growth to that in BM [BM(16-8)], but these two biomass had different biochemical compositions, and the former one had lower carbohydrate and higher protein content. When cultured in domestic wastewater, a higher biomass was obtained under continuous illumination [DW(24-0)], and the cells had higher carbohydrate and lower protein concentrations than that of DW(16-8). The spectra of the Fourier transform infrared spectrometer revealed that the functional groups on the surface of the three kinds of biomass--DW(16-8), DW(24-0), and BM(16-8)--were comparable, except an additional peak at 1731 cm(-1) was found in the biomass cultured in domestic wastewater, which was probably the result of bacterial contamination. Although biochemical differences were found among the three kinds of microalgal biomass, similar biosorption performances to chromium pollutants were recorded, with approximately 75% Cr(III) and 100% Cr(VI) removed at equilibrium in Cr(III) and Cr(VI) experiments, respectively, when dead biomass was used as a biosorbent. Therefore, it is possible to culture Chlorella miniata in domestic wastewater and use the biomass for the removal of chromium pollutants.
将小球藻（Chlorella miniata）在生活污水（DW）培养物中获取和利用其生物质用于去除铬（III）[Cr（III）]和铬（VI）[Cr（VI）]的可行性与在商业布里斯托尔培养基（BM）中的情况进行了比较。结果表明，在16 - 8小时光暗循环条件下（[DW(16 - 8)]）培养的小球藻在生活污水中的生长情况与在BM（[BM(16 - 8)]）中的相似，但这两种生物质具有不同的生化组成，前者碳水化合物含量较低而蛋白质含量较高。当在生活污水中培养时，在连续光照条件下（[DW(24 - 0)]）可获得更高的生物质，且细胞中的碳水化合物浓度高于DW(16 - 8)，而蛋白质浓度低于DW(16 - 8)。傅里叶变换红外光谱仪的光谱显示，三种生物质——DW(16 - 8)、DW(24 - 0)和BM(16 - 8)——表面的官能团具有可比性，只是在生活污水中培养的生物质中发现了一个位于1731 cm(-1)的额外峰，这可能是细菌污染的结果。尽管在三种微藻生物质之间发现了生化差异，但它们对铬污染物的生物吸附性能相似，当使用死生物质作为生物吸附剂时，在Cr(III)和Cr(VI)实验中，平衡时分别去除了约75%的Cr(III)和100%的Cr(VI)。因此，在生活污水中培养小球藻并利用其生物质去除铬污染物是可行的。
