从蛋白质消化物中去除去污剂以进行质谱分析。
Removal of detergents from protein digests for mass spectrometry analysis.
作者信息
Yeung Yee-Guide, Nieves Edward, Angeletti Ruth Hogue, Stanley E Richard
机构信息
Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA.
出版信息
Anal Biochem. 2008 Nov 15;382(2):135-7. doi: 10.1016/j.ab.2008.07.034. Epub 2008 Aug 3.
Abstract
Detergents are commonly used for the extraction of hydrophobic proteins and must be removed for sensitive detection of peptides by mass spectrometry. We demonstrate that ethyl acetate is able to extract octylglycoside from a protease digest without loss of peptides or interference with the peptide mass spectral profile. Ethyl acetate extraction was also found to reduce interference by sodium dodecyl sulfate, Nonidet P-40, or Triton X-100 in the mass spectrometry analysis.
摘要
去污剂通常用于提取疏水蛋白，而在通过质谱对肽段进行灵敏检测时必须将其去除。我们证明，乙酸乙酯能够从蛋白酶消化产物中提取辛基糖苷，而不会损失肽段或干扰肽段的质谱图。还发现乙酸乙酯萃取可减少十二烷基硫酸钠、Nonidet P - 40或Triton X - 100在质谱分析中的干扰。
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