德国小蠊中σ和δ类谷胱甘肽S-转移酶的变应原性。
Allergenicity of sigma and delta class glutathione S-transferases from the German cockroach.
作者信息
Jeong Kyoung Yong, Jeong Kyoung-jin, Yi Myung-hee, Lee Haeseok, Hong Chein-Soo, Yong Tai-Soon
机构信息
Department of Environmental Medical Biology and Institute of Tropical Medicine, Arthropods of Medical Importance Resource Bank, Yonsei University College of Medicine, Seoul, Korea.
出版信息
Int Arch Allergy Immunol. 2009;148(1):59-64. doi: 10.1159/000151506. Epub 2008 Aug 21.
BACKGROUND
Cockroach glutathione S-transferases (GSTs) are known to elicit strong IgE responses. This study was undertaken to compare the IgE reactivity of German cockroach GSTs, Bla g 5 (sigma class) and delta class GST (BgGSTD1).
METHODS
Full-length Bla g 5 and BgGSTD1 were cloned, and their recombinant proteins were expressed and purified. Their IgE reactivities and cross-reactivities were examined by ELISA using sera from cockroach-sensitized subjects.
RESULTS
A predominant variant of Bla g 5 cDNA has amino acid substitutions at positions 10 (C to F) and 42 (N to K). BgGSTD1 has substitutions at positions 27 (E to N) and 207 (K to R). Sera from cockroach-sensitized patients showed 20.5% IgE reactivity to Bla g 5 and 17.9% IgE reactivity to BgGSTD1. However, inhibition studies using 1 serum sample with the highest IgE reactivity showed limited cross-reactivity.
CONCLUSIONS
IgE-binding frequency to the cockroach GSTs was low, but the titer of IgE reactivity was strong in some sera. The inclusion of different classes of GSTs could be helpful for the delicate diagnosis and immunotherapy of cockroach allergy.
背景
已知蟑螂谷胱甘肽S-转移酶（GSTs）会引发强烈的IgE反应。本研究旨在比较德国蟑螂GSTs、Bla g 5（sigma类）和delta类GST（BgGSTD1）的IgE反应性。
方法
克隆全长Bla g 5和BgGSTD1，并表达和纯化其重组蛋白。使用蟑螂致敏受试者的血清通过ELISA检测它们的IgE反应性和交叉反应性。
结果
Bla g 5 cDNA的一个主要变体在第10位（C到F）和第42位（N到K）有氨基酸替换。BgGSTD1在第27位（E到N）和第207位（K到R）有替换。蟑螂致敏患者的血清对Bla g 5的IgE反应性为20.5%，对BgGSTD1的IgE反应性为17.9%。然而，使用1份IgE反应性最高的血清样本进行的抑制研究显示交叉反应性有限。
结论
蟑螂GSTs的IgE结合频率较低，但在一些血清中IgE反应性滴度较强。纳入不同类别的GSTs可能有助于蟑螂过敏的精细诊断和免疫治疗。
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