Satinover Shama M, Reefer Amanda J, Pomes Anna, Chapman Martin D, Platts-Mills Thomas A E, Woodfolk Judith A
Asthma and Allergic Diseases Center, University of Virginia Health System, Charlottesville, VA 22908-1355, USA.
J Allergy Clin Immunol. 2005 Apr;115(4):803-9. doi: 10.1016/j.jaci.2005.01.018.
The specificity of serum antibody responses to different cockroach allergens has not been studied.
We sought to quantitate serum IgE and IgG antibodies to a panel of purified cockroach allergens among cockroach-sensitized subjects.
IgE antibodies to recombinant cockroach allergens (rBla g 1, rBla g 2, rBla g 4, rBla g 5, and rPer a 7) were measured in sera containing IgE antibodies to Blattella germanica extract (n = 118) by using a streptavidin CAP assay and a multiplex flow cytometric assay. Specific IgG antibodies were determined by using radioimmunoprecipitation techniques.
Specific IgE antibodies measured by means of CAP assay and multiplex assay were strongly correlated ( r = 0.8, P < .001). The sum of IgE antibodies (in international units per milliliter) against all 5 allergens equated to IgE antibodies to cockroach extract. Although the prevalence of IgE antibodies was highest for rBla g 2 (54.4%) and rBla g 5 (37.4%), patterns of IgE antibody binding were unique to each subject. Surprisingly, only 16% of cockroach-sensitized subjects with IgE antibodies to house dust mite exhibited IgE antibody binding to cockroach tropomyosin (rPer a 7). Specific IgE antibodies were associated with increased IgG antibody levels, although detection of IgG in the absence of IgE was not uncommon.
The techniques described offer a new approach for defining the hierarchy of purified allergens. IgE antibodies directed against 5 allergens constitute the majority of the IgE antibody repertoire for cockroach. Such distinct patterns of IgE-IgG responsiveness to different cockroach allergens highlight the complexity of B-cell responses to environmental allergens.
尚未研究血清抗体对不同蟑螂过敏原反应的特异性。
我们试图对蟑螂致敏受试者中一组纯化蟑螂过敏原的血清IgE和IgG抗体进行定量。
通过链霉亲和素CAP检测和多重流式细胞术检测，在含有针对德国小蠊提取物的IgE抗体的血清（n = 118）中测量针对重组蟑螂过敏原（rBla g 1、rBla g 2、rBla g 4、rBla g 5和rPer a 7）的IgE抗体。使用放射免疫沉淀技术测定特异性IgG抗体。
通过CAP检测和多重检测测得的特异性IgE抗体高度相关（r = 0.8，P <.001）。针对所有5种过敏原的IgE抗体总和（以每毫升国际单位计）等同于针对蟑螂提取物的IgE抗体。尽管rBla g 2（54.4%）和rBla g 5（37.4%）的IgE抗体阳性率最高，但每个受试者的IgE抗体结合模式都是独特的。令人惊讶的是，在对屋尘螨有IgE抗体的蟑螂致敏受试者中，只有16%表现出与蟑螂原肌球蛋白（rPer a 7）的IgE抗体结合。特异性IgE抗体与IgG抗体水平升高相关，尽管在没有IgE的情况下检测到IgG并不罕见。
所描述的技术为确定纯化过敏原的等级提供了一种新方法。针对5种过敏原的IgE抗体构成了蟑螂IgE抗体库的大部分。这种对不同蟑螂过敏原的IgE - IgG反应性的独特模式突出了B细胞对环境过敏原反应的复杂性。
