Pomés Anna, Arruda Luisa Karla
Indoor Biotechnologies, Inc., Charlottesville, VA, USA.
School of Medicine of Ribeirao Preto, University of Sao Paulo, Brazil.
Methods. 2014 Mar 1;66(1):75-85. doi: 10.1016/j.ymeth.2013.07.036. Epub 2013 Aug 2.
Cockroach allergy is an important health problem associated with the development of asthma, as a consequence of chronic exposure to low levels of allergens in susceptible individuals. In the last 20 years, progress in understanding the disease has been possible, thanks to the identification and molecular cloning of cockroach allergens and their expression as recombinant proteins. Assays for assessment of environmental allergen exposure have been developed and used to measure Bla g 1 and Bla g 2, as markers of cockroach exposure. IgE antibodies to cockroach extracts and to specific purified allergens have been measured to assess sensitization and analyze association with exposure and disease. With the development of the field of structural biology and the expression of recombinant cockroach allergens, insights into allergen structure, function, epitope mapping and allergen-antibody interactions have provided further understanding of mechanisms of cockroach allergic disease at the molecular level. This information will contribute to develop new approaches to allergen avoidance and to improve diagnosis and therapy of cockroach allergy.
蟑螂过敏是一个与哮喘发展相关的重要健康问题，这是易感个体长期接触低水平过敏原的结果。在过去20年里，由于蟑螂过敏原的鉴定、分子克隆以及它们作为重组蛋白的表达，在了解该疾病方面取得了进展。已开发出评估环境过敏原暴露的检测方法，并用于测量Bla g 1和Bla g 2，作为蟑螂暴露的标志物。已测量针对蟑螂提取物和特定纯化过敏原的IgE抗体，以评估致敏情况并分析其与暴露和疾病的关联。随着结构生物学领域的发展以及重组蟑螂过敏原的表达，对过敏原结构、功能、表位图谱和过敏原 - 抗体相互作用的深入了解，在分子水平上进一步加深了对蟑螂过敏性疾病机制的认识。这些信息将有助于开发新的避免接触过敏原的方法，并改善蟑螂过敏的诊断和治疗。
