Sakamoto T, Hiyama Y
Division of Drugs, National Institute of Health Sciences, Tokyo, Japan.
Pharmazie. 2008 Jul;63(7):503-7.
A simple and rapid determination method for nitazoxanide (NTZ), an antiprotozoal agent, was developed using reverse-phase HPLC and Ultra Performance Liquid Chromatography (UPLC). Only six minutes gradient condition for NTZ analysis using UPLC was achieved. The mobile phase consisted of a mixture of phosphate buffer (pH 6.0) and acetonitrile. The repeatability (relative standard deviation (RSD), n = 6) and the correlation coefficient from linearity (the range from 80% to 120% of amount) were 0.25% and 0.9963 for UPLC and 0.15% and 0.9988 for HPLC, respectively. The quantitative values of NTZ in tablets were 103.2% for HPLC and 98.7% for UPLC. The RSDs of quantitative values of sample solution were calculated to be 4.06% to 4.64% for HPLC and 0.15% to 0.36% for UPLC.
利用反相高效液相色谱法（HPLC）和超高效液相色谱法（UPLC）开发了一种用于抗寄生虫药物硝唑尼特（NTZ）的简单快速测定方法。使用UPLC对NTZ进行分析时，仅需6分钟的梯度条件。流动相由磷酸盐缓冲液（pH 6.0）和乙腈的混合物组成。UPLC的重复性（相对标准偏差（RSD），n = 6）和线性相关系数（量的80%至120%范围）分别为0.25%和0.9963，HPLC分别为0.15%和0.9988。片剂中NTZ的定量值，HPLC为103.2%，UPLC为98.7%。样品溶液定量值的RSD，HPLC计算为4.06%至4.64%，UPLC为0.15%至0.36%。
