Malik Ashok Kumar, Rai Pramod Kumar
Department of Chemistry, Punjabi University, Patiala-147002, Punjab, India.
J Chromatogr Sci. 2008 Aug;46(7):609-14. doi: 10.1093/chromsci/46.7.609.
A method for enhanced extraction of octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX) and hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) from environmental samples is developed with the assistance of sodium dodecyl sulphate (SDS) surfactant. In this study, the concentration of SDS surfactant and other analytical parameters are optimized on a high-performance liquid chromatography-UV system. An isocratic flow of 1.0 mL/min with mobile phase acetonitrile-water; 70:30 (v/v) at 230 nm wavelength on a reverse-phase amide column is used for baseline separation of explosives and making calibration curves. The amount of recovered explosives from spiked soil and water samples are calculated. The limits of detection obtained for HMX and RDX standards are 1.5 and 3.8 ppb (S/N=3), respectively, which are much better than obtained by the Environmental Protection Agency method 8330. The recoveries are found to be enhanced by 1.7 and 1.6-fold with SDS solution as compared to water for HMX and RDX, respectively, from soil samples.
在十二烷基硫酸钠（SDS）表面活性剂的辅助下，开发了一种从环境样品中增强提取八氢-1,3,5,7-四硝基-1,3,5,7-四氮杂环辛烷（HMX）和六氢-1,3,5-三硝基-1,3,5-三嗪（RDX）的方法。在本研究中，在高效液相色谱-紫外系统上对SDS表面活性剂的浓度和其他分析参数进行了优化。在反相酰胺柱上，以1.0 mL/min的等度流速，流动相为乙腈-水（70:30，v/v），在230 nm波长下用于炸药的基线分离并制作校准曲线。计算了从加标土壤和水样中回收的炸药量。HMX和RDX标准品的检测限分别为1.5和3.8 ppb（S/N = 3），比美国环境保护局方法8330获得的检测限要好得多。与水相比，对于土壤样品中的HMX和RDX，SDS溶液的回收率分别提高了1.7倍和1.6倍。
