García-Peláez Beatriz, Vilà Ruth, Remesar Xavier
Departament de Nutrició i Bromatologia, Universitat de Barcelona, Instituto de Salud Carlos III, Barcelona, Spain.
Obesity (Silver Spring). 2008 Oct;16(10):2223-31. doi: 10.1038/oby.2008.341. Epub 2008 Jul 17.
To determine whether treatment of rat dams with oleoyl-estrone (OE) has an effect on the offspring's long-term response to diet restriction during lactation.
Control, OE-treated, and diet-restricted dams were treated up to day 15 of lactation. Changes in food intake and body weight were recorded for dams and their pups. After weaning, pups received a 4-week standard diet followed by a 4-week period of high-fat diet. Lipid, protein, and energy content of pups plus energy intake and efficiency. Serum metabolites (glucose, urea, and cholesterol) and serum hormones (adiponectin, leptin, insulin, and sexual hormones).
Neither pups from dams in the OE-treated nor in the diet-restricted group showed significant changes in weight, though these two groups ingested 79% of food ingested by controls. At weaning, the pups from OE-treated rats were smaller than those of the control or diet-restricted groups. These pups maintained the differences in size and lipid content during the 4-week standard-diet period, whereas pups from diet-restricted dams showed a sharp decrease in their lipid content. During the 4 weeks of high-fat diet, the male offspring from OE-treated dams increased the difference in lipid content in relation to the pups from control dams whereas in females the differences decreased. Female offspring from diet-restricted dams showed the most marked changes in metabolite and hormone levels in relation to controls.
Treatment of lactating dams with OE programs the metabolic response of their offspring to resist the challenge of a high-fat diet that would lead to obesity in adulthood.
确定用油酰雌酮（OE）处理大鼠母鼠是否会对其后代在哺乳期对饮食限制的长期反应产生影响。
对照组、经OE处理组和饮食限制组的母鼠在哺乳期第15天前接受处理。记录母鼠及其幼崽的食物摄入量和体重变化。断奶后，幼崽先接受4周的标准饮食，随后是4周的高脂饮食。测定幼崽的脂质、蛋白质和能量含量以及能量摄入和效率。检测血清代谢物（葡萄糖、尿素和胆固醇）和血清激素（脂联素、瘦素、胰岛素和性激素）。
经OE处理组和饮食限制组母鼠的幼崽体重均无显著变化，不过这两组幼崽的食物摄入量为对照组的79%。断奶时，经OE处理的大鼠幼崽比对照组或饮食限制组的幼崽体型小。在4周的标准饮食期内，这些幼崽在体型和脂质含量上保持差异，而饮食限制组母鼠的幼崽脂质含量急剧下降。在4周的高脂饮食期，经OE处理组母鼠的雄性后代与对照组母鼠的幼崽相比，脂质含量差异增大，而雌性后代的差异减小。饮食限制组母鼠的雌性后代与对照组相比，代谢物和激素水平变化最为明显。
用OE处理哺乳期母鼠会使其后代产生代谢反应，以抵抗高脂饮食的挑战，否则这种饮食会导致成年后肥胖。
