T32-ISTRATI福氏志贺菌2a疫苗株的毒力表型及遗传特征

Virulence phenotype and genetic characteristics of the T32-ISTRATI Shigella flexneri 2a vaccine strain.

作者信息

Venkatesan M, Fernandez-Prada C, Buysse J M, Formal S B, Hale T L

机构信息

Department of Bacterial Immunology, Walter Reed Army Institute of Research, Washington, DC 20307.

出版信息

Vaccine. 1991 May;9(5):358-63. doi: 10.1016/0264-410x(91)90064-d.

DOI:10.1016/0264-410x(91)90064-d

PMID:1872021

Abstract

The T32-ISTRATI strain, which has been used as an oral attenuated Shigella flexneri 2a vaccine, has lost the invasive phenotype due to a spontaneous deletion in the shigella virulence plasmid. This deletion has eliminated three plasmid loci (ipaBCDA, invA and virG) that are necessary for production of a positive Sereny test by Shigella species. Virulence in the Sereny test was reconstituted in the T32-ISTRATI strain by the conjugal transfer of an intact 140 M Da virulence plasmid from S. flexneri 5. The T32-ISTRATI vaccine is safe when given orally in multiple doses of 50-100 x 10(9) organisms, and both homologous and heterologous protection has been reported in large Romanian and Chinese field trials. Although the protective antigen(s) in this vaccine have not been identified, the potential use of non-invasive plasmid deletion mutants as living shigella vaccines is illustrated by the T32-ISTRATI vaccine.

摘要

T32-ISTRATI菌株曾用作口服减毒福氏志贺菌2a疫苗，由于志贺菌毒力质粒的自发缺失，该菌株已失去侵袭表型。这种缺失消除了志贺菌属产生阳性塞雷尼试验所必需的三个质粒位点（ipaBCDA、invA和virG）。通过从福氏志贺菌5型进行完整的140 M Da毒力质粒的接合转移，在T32-ISTRATI菌株中重建了塞雷尼试验中的毒力。当以50-100×10⁹个菌体的多剂量口服时，T32-ISTRATI疫苗是安全的，并且在罗马尼亚和中国的大型现场试验中均报道了同源和异源保护作用。尽管该疫苗中的保护性抗原尚未确定，但T32-ISTRATI疫苗说明了非侵袭性质粒缺失突变体作为活志贺菌疫苗的潜在用途。