减毒的缺失aroA和缺失virG的福氏志贺菌2a菌株CVD 1203（一种口服活疫苗原型）的构建与特性分析
Construction and characterization of attenuated delta aroA delta virG Shigella flexneri 2a strain CVD 1203, a prototype live oral vaccine.
作者信息
Noriega F R, Wang J Y, Losonsky G, Maneval D R, Hone D M, Levine M M
机构信息
Department of Pediatrics, University of Maryland School of Medicine, Baltimore 21201.
出版信息
Infect Immun. 1994 Nov;62(11):5168-72. doi: 10.1128/iai.62.11.5168-5172.1994.
Abstract
We engineered an oral Shigella vaccine prototype that can invade intestinal epithelial cells but cannot undergo extensive intracellular replication or extend to adjacent epithelial cells. Strain CVD 1203, derived from wild-type Shigella flexneri 2a by introducing deletions in chromosomal aroA and invasion plasmid virG, was highly attenuated in the Sereny test. Two 10(9)-CFU orogastric doses (2 weeks apart) stimulated production of secretory immunoglobulin A antibodies to S. flexneri 2a and protected against conjunctival sac challenge with virulent S. flexneri 2a.
摘要
我们构建了一种口服志贺氏菌疫苗原型，它能够侵入肠道上皮细胞，但无法进行广泛的细胞内复制或扩散至相邻上皮细胞。菌株CVD 1203由野生型福氏志贺氏菌2a通过在染色体aroA和侵袭质粒virG中引入缺失而获得，在塞雷尼试验中高度减毒。两次口服剂量为10⁹CFU（间隔2周）可刺激产生针对福氏志贺氏菌2a的分泌型免疫球蛋白A抗体，并保护机体免受强毒性福氏志贺氏菌2a的结膜囊攻击。
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Construction and characterization of attenuated delta aroA delta virG Shigella flexneri 2a strain CVD 1203, a prototype live oral vaccine.减毒的缺失aroA和缺失virG的福氏志贺菌2a菌株CVD 1203（一种口服活疫苗原型）的构建与特性分析
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