以色列志贺氏菌临床分离株中志贺氏菌肠毒素1和2基因的流行情况。
Prevalence of the genes for shigella enterotoxins 1 and 2 among clinical isolates of shigella in Israel.
作者信息
Yavzori M, Cohen D, Orr N
机构信息
Army Health Branch Research Unit, Medical Corps, Israel Defense Force.
出版信息
Epidemiol Infect. 2002 Jun;128(3):533-5. doi: 10.1017/s0950268802006866.
Two enterotoxins, shigella enterotoxin 1 (SHET1) and shigella enterotoxin 2 (SHET2) have been recently characterized and are believed to play a role in the clinical manifestation of shigellosis. One hundred and twenty-one isolates of Shigella spp. of 13 different serotypes and variants and 10 isolates of enteroinvasive Escherichia coli (EIEC) isolated in Israel, were examined by polymerase chain reaction for the presence of SHET1 and SHET2 genes. SHET1 was only prevalent among isolates of S. flexneri 2a while SHET2 was found in all the serotypes that were tested except for several isolates of S. flexneri 1b that lost their virulence plasmid during storage. In addition, we found that the S. flexneri 2a vaccine strain T-32 Istrati contains the gene encoding for SHET1 but not that encoding for SHET2, suggesting that the latter is located within a large deletion occurring in the 140 Mda plasmid of this S. flexneri 2a non-invasive vaccine strain.
最近已鉴定出两种肠毒素，即志贺氏菌肠毒素1（SHET1）和志贺氏菌肠毒素2（SHET2），据信它们在志贺氏菌病的临床表现中发挥作用。对以色列分离出的13种不同血清型和变种的121株志贺氏菌属菌株以及10株侵袭性大肠杆菌（EIEC）进行聚合酶链反应检测，以确定是否存在SHET1和SHET2基因。SHET1仅在福氏志贺氏菌2a菌株中流行，而SHET2在所有测试血清型中均有发现，但有几株福氏志贺氏菌1b菌株在储存过程中失去了毒力质粒除外。此外，我们发现福氏志贺氏菌2a疫苗株T - 32 Istrati含有编码SHET1的基因，但不含有编码SHET2的基因，这表明后者位于该福氏志贺氏菌2a非侵袭性疫苗株140 Mda质粒的一个大缺失区域内。
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