[栀子炮制前后环烯醚萜类成分含量的比较研究]
[Comparative studies on contents of iridoid in processed products from Gardenia jasminoides].
作者信息
Zhang Cun, Xiao Yong-Qing, Li Li, Pang Zhen, Li Gui-Liu
机构信息
Institute of Chinese Materia Madica, China Academy of Chinese Medicial Sciences, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2008 May;33(10):1138-40.
OBJECTIVE
To compare the contents of iridoid in the different processed products from Gardenia jasminoides.
METHOD
Contents of geniposide and genipin gentiobioside in products were determined simultaneously by HPLC. A kromasil C18 column at 35 degrees C was used with the acetonitrile-0.3% formic acid anhydrous (12:88) as the mobile phrase. The detection wavelength was set at 238 nm and the flow rate was 1.0 mL x min(-1).
RESULT
The obtained linearity of the two components was good with that the relative coefficients (r) were over 0.9994 and the average recoveries were 101.8%, 99.1%, respectively.
CONCLUSION
The method is simple, accurate with good reproducibility. The results showed contents of two components are difference in the different processed products.
目的
比较栀子不同炮制品中环烯醚萜苷的含量。
方法
采用高效液相色谱法同时测定制品中栀子苷和京尼平龙胆双糖苷的含量。使用kromasil C18柱，柱温35℃，以乙腈-0.3%无水甲酸（12:88）为流动相。检测波长设定为238nm，流速为1.0mL·min⁻¹。
结果
两种成分的线性关系良好，相关系数（r）均大于0.9994，平均回收率分别为101.8%、99.1%。
结论
该方法简便、准确，重现性好。结果表明不同炮制品中两种成分的含量存在差异。
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