[反相高效液相色谱法快速同时测定清胃黄连片中4种指标成分]
[Fast and simultaneous determination of four index components in Qingwei Huanglian tablets by RP-HPLC].
作者信息
Li Yan-Rong, Jiang Ye, Hao Fu, Ding Xiang-Yu
机构信息
School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2007 Dec;32(24):2597-600.
OBJECTIVE
To establish a RP-HPLC method for fast and simultaneous determination of four index contents, which are Genpioside, Baicalin, Berberine Hydrochloride and Ammonium Glyeyrrhizinate, in Qingwei Huanglian tablets.
METHOD
A separation was well achieved by gradient elution on a Hypersil C18 (4.6 mm x 250 mm, 5 microm) column with the mobile phases of acetontrile -0.5% triethylamine water-solution (pH 3.0) at a flow rate of 1.0 mL x min(-1), detection wavelength of 230 nm, and room temperature.
RESULT
The calibration curve were linear in the ranges of 4.82-77.2, 5.80-92.8, 1.63-26.1 and 6.40-102 microg x mL(-1) (r = 0.9997-0.9999) for genpioside, baicalin, berberine hydrochloride and ammonium glyeyrrhizinate, respectively. The average recoveries of four index components were not less than 98.0%.
CONCLUSION
The method is convenient, rapid and accurate. It is suitable for the quality control of Qingwei Huanglian tablets.
目的
建立一种反相高效液相色谱法，用于快速同时测定清胃黄连片中的4种指标成分，即栀子苷、黄芩苷、盐酸小檗碱和甘草酸铵。
方法
采用Hypersil C18（4.6 mm×250 mm，5μm）色谱柱，以乙腈-0.5%三乙胺水溶液（pH 3.0）为流动相进行梯度洗脱，流速为1.0 mL·min⁻¹，检测波长为230 nm，柱温为室温，实现了良好的分离。
结果
栀子苷、黄芩苷、盐酸小檗碱和甘草酸铵的校准曲线分别在4.82 - 77.2、5.80 - 92.8、1.63 - 26.1和6.40 - 102μg·mL⁻¹范围内呈线性关系（r = 0.9997 - 0.9999）。4种指标成分的平均回收率均不低于98.0%。
结论
该方法简便、快速、准确，适用于清胃黄连片的质量控制。
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