Yuan N, Pitts D G
College of Optometry, University of Houston, Texas.
Acta Ophthalmol (Copenh). 1991 Apr;69(2):241-6. doi: 10.1111/j.1755-3768.1991.tb02718.x.
The purpose of the study was to investigate the effect of H2O2 on corneal swelling, corneal deswelling and to determine the concentration of H2O2 in the aqueous humor. Four types of experiments were performed using the rabbit eye. 1. Central corneal thickness (CCT) measurements after instillation of H2O2 into the cul-de-sac and after wearing H2O2 soaked soft contact lenses (SLC) for 2 h using 60 ppm, 100 ppm and 300 ppm H2O2. 2. Measurement of corneal deswelling induced by wearing SCL and closed eye for 2 h. 3. Determining the H2O2 in the aqueous humor one h after instillation of H2O2 into the cul-de-sac. CCT increases of 7.5% and 10.7% were found after the instillation of 100 ppm and 300 ppm H2O2 into the cul-de-sac. After wearing H2O2 soaked SCLs, CCT increased 4.6% and 5.8%, respectively. The H2O2 in the aqueous humor was 0.087 mM or a 160% increase after using 300 ppm H2O2. The increase in CCT and interference with the deswelling process is interpreted as a toxic effect on the cornea and an interference with the normal dehydration function of the endothelial pump.
本研究的目的是调查过氧化氢（H2O2）对角膜肿胀、角膜消肿的影响，并确定房水中H2O2的浓度。使用兔眼进行了四种类型的实验。1. 向结膜囊滴入H2O2以及佩戴浸泡过H2O2的软性隐形眼镜（SLC）2小时后，分别使用60 ppm、100 ppm和300 ppm的H2O2测量中央角膜厚度（CCT）。2. 测量佩戴SCL并闭眼2小时引起的角膜消肿情况。3. 向结膜囊滴入H2O2一小时后测定房水中的H2O2。向结膜囊滴入100 ppm和300 ppm的H2O2后，发现CCT分别增加了7.5%和10.7%。佩戴浸泡过H2O2的SCL后，CCT分别增加了4.6%和5.8%。使用300 ppm的H2O2后，房水中的H2O2为0.087 mM，即增加了160%。CCT的增加以及对消肿过程的干扰被解释为对角膜的毒性作用以及对内皮泵正常脱水功能的干扰。
