Konishi Y, Kawabata M
Department of Ophthalmology, School of Medicine, Tokushima University, Japan.
Nippon Ganka Gakkai Zasshi. 1991 May;95(5):462-7.
In a previous study, IgG deposits and changes were found in the corneal epithelial cells of BALB/c mice immunized by cornea-specific protein (CSP) contained in the bovine cornea. We observed the corneal changes in mice by immunoreaction to CSP for 6 months with slit-lamp microscopy, and found granular opacity under the central corneal epithelium. Histologically this opacity was composed of scattered deposits in the corneal substantia beneath the basal layer of the epithelium. The corneal epithelium in contact with the deposits became atrophied and flattened. The deposits were stained with PAS staining, and with ConA, WGA and RCA by the lectin enzyme histochemical method. Therefore, these results indicate that these deposits possess N-acetyl-D-glucosamine as a sugar residue. In the degenerated corneal epithelium, peroxidase-staining by the anti-CSP antibody enzyme histochemical method weakened and ConA-positive cells were obviously decreased. This suggests that the production of CSP decreased in the same lesion and that disorder of sugar metabolism developed at the corneal epithelium.
在先前的一项研究中，在经牛角膜中所含角膜特异性蛋白（CSP）免疫的BALB/c小鼠的角膜上皮细胞中发现了IgG沉积和变化。我们用裂隙灯显微镜观察了小鼠对CSP免疫反应6个月的角膜变化，发现在角膜中央上皮下方有颗粒状混浊。组织学上，这种混浊由上皮基底层下方角膜基质中的散在沉积物组成。与沉积物接触的角膜上皮萎缩并变平。沉积物经PAS染色以及通过凝集素酶组织化学方法用伴刀豆球蛋白A（ConA）、麦胚凝集素（WGA）和蓖麻凝集素（RCA）染色。因此，这些结果表明这些沉积物含有N-乙酰-D-葡萄糖胺作为糖残基。在变性的角膜上皮中，抗CSP抗体酶组织化学方法的过氧化物酶染色减弱，ConA阳性细胞明显减少。这表明在同一病变中CSP的产生减少，并且角膜上皮出现了糖代谢紊乱。
