Prabakaran G, Hoti L
Vector Control Research Centre (Indian Council of Medical Research), Indira Nagar, Puducherry 605006, India. gprabha
Acta Trop. 2008 Oct;108(1):50-3. doi: 10.1016/j.actatropica.2008.07.007. Epub 2008 Aug 5.
Bacillus sphaericus has been widely used in mosquito control programs, but the production of this bacterium is a little tricky as it does not utilize carbohydrates and requires proteinaceous substrates, which are expensive. In this study, we developed a cost-effective medium that resulted in a lower cost and shorter fermentation time. The locally available raw material, egg yolk was used and the level of sporulation, toxicity and biomass were compared with the conventional medium. Use of the egg yolk culture medium significantly shortened fermentation time to 15 h and yielded high activity, equivalent to that of conventional medium against 3rd instar Culex quinquefasciatus. Conventional NYSM medium required 21 h to attain the maximum activity and biomass. Hence, the egg yolk-based culture medium appears to be suitable and economical for the small-scale production of B. sphaericus.
球形芽孢杆菌已广泛应用于蚊虫控制项目，但这种细菌的生产有点棘手，因为它不利用碳水化合物，需要蛋白质类底物，而这类底物价格昂贵。在本研究中，我们开发了一种具有成本效益的培养基，其成本更低且发酵时间更短。使用了当地可得的原材料蛋黄，并将其芽孢形成水平、毒性和生物量与传统培养基进行了比较。使用蛋黄培养基可显著将发酵时间缩短至15小时，并产生高活性，与传统培养基对致倦库蚊三龄幼虫的活性相当。传统的NYSM培养基需要21小时才能达到最大活性和生物量。因此，基于蛋黄的培养基似乎适合且经济用于球形芽孢杆菌的小规模生产。
