椰子水作为一种廉价来源，用于生产苏云金芽孢杆菌以色列变种的δ内毒素，一种灭蚊剂。

Coconut water as a cheap source for the production of delta endotoxin of Bacillus thuringiensis var. israelensis, a mosquito control agent.

作者信息

Prabakaran G, Hoti S L, Manonmani A M, Balaraman K

机构信息

Vector Control Research Centre (Indian Council of Medical Research), Indira Nagar, Puducherry 605006, India.

出版信息

Acta Trop. 2008 Jan;105(1):35-8. doi: 10.1016/j.actatropica.2007.09.002. Epub 2007 Sep 20.

DOI:10.1016/j.actatropica.2007.09.002

PMID:17963708

Abstract

Bacillus thuringiensis var. israelensis (B. t. i.) is being widely used in mosquito control programs. However, the large-scale production of this bacillus is expensive due to the high cost of the production medium. In this study, we attempted to develop a cost-effective medium, based on a locally available raw material namely coconut water which is available in plenty as waste product from coconut oil industry. The yield of cell mass, sporulation and mosquito larvicidal activity were studied by growing this bacterium in this waste product and in comparison with the conventional medium (NYSM). Cell mass yield of 3.1g/L, spore count of 3.4x10(11)spores/mL and mosquito larvicidal activity (LC(50)) of 14.85ng/mL (against early fourth-instar larvae of Aedes aegypti) were obtained with a 30h old culture of this bacterium grown in coconut water. This is almost similar to that obtained with NYSM medium. Hence, coconut water-based culture medium is economical for the production of B. t. i.

摘要

苏云金芽孢杆菌以色列变种（B. t. i.）正被广泛用于蚊虫控制项目。然而，由于生产培养基成本高昂，这种芽孢杆菌的大规模生产费用不菲。在本研究中，我们尝试基于一种当地可得的原材料——椰子水来开发一种经济高效的培养基，椰子水作为椰子油工业的废品大量存在。通过在这种废品以及与传统培养基（NYSM）中培养该细菌，研究了细胞质量产量、孢子形成及蚊虫杀幼虫活性。用在椰子水中培养30小时的该细菌培养物，获得了细胞质量产量为3.1克/升、孢子计数为3.4×10¹¹个孢子/毫升以及蚊虫杀幼虫活性（LC₅₀）为14.85纳克/毫升（针对埃及伊蚊四龄初期幼虫）的结果。这几乎与在NYSM培养基中获得的结果相似。因此，基于椰子水的培养基对于生产B. t. i.来说是经济实惠的。