Dorrell Craig, Erker Laura, Lanxon-Cookson Kelsea M, Abraham Stephanie L, Victoroff Tristan, Ro Simon, Canaday Pamela S, Streeter Philip R, Grompe Markus
Oregon Health & Science University and the Oregon Stem Cell Center, Portland, OR 97239, USA.
Hepatology. 2008 Oct;48(4):1282-91. doi: 10.1002/hep.22468.
The biology of progenitor activation in the liver is of considerable medical and scientific interest. The powerful genetic tools available for the mouse make it an ideal model system to study this complex process involving many different cell types. However, reagents for the isolation and study of distinct hepatic subpopulations have been quite limited compared to those available for hematopoietic cells. To produce cell surface reactive reagents more specific for the oval cell response, we generated a new collection of monoclonal antibodies by immunization of Fischer rats with enzymatically dispersed nonparenchymal cells from the livers of adult mice treated with 3,5-diethoxycarbonyl-1,4-dihydrocollidine. Each of the resulting antibodies recognized a surface antigen present on a liver cell subset and permitted the viable isolation of the associated subpopulation by fluorescence-activated cell sorting. Differential activity was observed on normal liver cells and at different stages of oval cell activation, indicating potential utility for progenitor cell identification. The subdivision of liver cells using these tools should facilitate the study of the biology of ductal and periductal hepatic cell types, including progenitors.
A new panel of surface reactive monoclonal antibodies to support investigation of the murine oval cell response has been developed.
肝脏中祖细胞激活的生物学具有重大的医学和科学研究价值。小鼠拥有强大的遗传工具，使其成为研究这一涉及多种不同细胞类型的复杂过程的理想模型系统。然而，与用于造血细胞的试剂相比，用于分离和研究不同肝亚群的试剂相当有限。为了制备对卵圆细胞反应更具特异性的细胞表面反应试剂，我们用经3,5 - 二乙氧基羰基 - 1,4 - 二氢可力丁处理的成年小鼠肝脏中的酶分散非实质细胞免疫Fischer大鼠，从而产生了一组新的单克隆抗体。所得的每种抗体都识别存在于肝细胞亚群上的一种表面抗原，并允许通过荧光激活细胞分选对相关亚群进行活细胞分离。在正常肝细胞和卵圆细胞激活的不同阶段观察到了差异活性，表明其在祖细胞鉴定方面具有潜在用途。使用这些工具对肝细胞进行细分应有助于研究导管和导管周围肝细胞类型（包括祖细胞）的生物学特性。
已开发出一组新的表面反应性单克隆抗体，以支持对小鼠卵圆细胞反应的研究。
