Ferraro A, Grandi P, Eufemi M, Altieri F, Cervoni L, Turano C
Department of Biochemical Sciences A. Rossi Fanelli, University La Sapienza, Rome.
Biochem Biophys Res Commun. 1991 Aug 15;178(3):1365-70. doi: 10.1016/0006-291x(91)91044-d.
The protein-DNA crosslinking capability of cis-dichloro diammineplatinum has been exploited to check the intranuclear location of N-glycosylated proteins. When intact liver cells were treated with this reagent, a number of glycoproteins, recognized by Concanavalin A, have been shown to become crosslinked to DNA; many of them have been recognized as nuclear matrix components. The recognition by this lectin was abolished by treatment with N-glycosidase F, showing the presence of N-glycosidic bonds between the sugar moiety and the protein. Most of the glycoproteins appeared to have high mannose oligosaccharide chains, but sialic acid containing oligosaccharides were also identified.
顺式二氯二氨铂的蛋白质 - DNA交联能力已被用于检测N - 糖基化蛋白在细胞核内的位置。当完整的肝细胞用该试剂处理时，已证明一些被伴刀豆球蛋白A识别的糖蛋白会与DNA交联；其中许多已被确认为核基质成分。用N - 糖苷酶F处理可消除这种凝集素的识别作用，表明糖部分与蛋白质之间存在N - 糖苷键。大多数糖蛋白似乎具有高甘露糖寡糖链，但也鉴定出了含唾液酸的寡糖。
