Kanatani M, Sugimoto T, Kano J, Fukase M, Fujita T
Department of Medicine, Kobe University School of Medicine, Japan.
Biochem Biophys Res Commun. 1991 Aug 15;178(3):866-70. doi: 10.1016/0006-291x(91)90971-9.
Effects of human monocyte-conditioned medium on the proliferation of osteoblastic MC3T3-E1 cells were investigated in serum-free cultured condition. Monocyte-conditioned medium significantly stimulated osteoblast proliferation at the concentration between 10 and 30%, compared to that in the absence of monocytes. 17 beta-estradiol directly stimulated osteoblast proliferation at the concentrations of 10(-8) and 10(-10)M. On the contrary, the conditioned medium prepared by monocytes cultured in the presence of 17 beta-estradiol at the concentrations of 10(-8) and 10(-10)M significantly inhibited osteoblast proliferation. Present data indicate that in addition to direct effect on osteoblasts, 17 beta-estradiol affected osteoblast proliferation presumably through modulating the release of several local regulators of bone turnover from monocytes. The effect on osteoblastic activity via monocytes might be linked to the coupling of osteoclast and osteoblast actions.
在无血清培养条件下，研究了人单核细胞条件培养基对成骨细胞MC3T3-E1细胞增殖的影响。与不存在单核细胞的情况相比，单核细胞条件培养基在浓度为10%至30%时显著刺激成骨细胞增殖。17β-雌二醇在10^(-8)和10^(-10)M浓度下直接刺激成骨细胞增殖。相反，在10^(-8)和10^(-10)M浓度的17β-雌二醇存在下培养的单核细胞制备的条件培养基显著抑制成骨细胞增殖。目前的数据表明，除了对成骨细胞的直接作用外，17β-雌二醇可能通过调节单核细胞释放几种骨转换局部调节因子来影响成骨细胞增殖。通过单核细胞对成骨细胞活性的影响可能与破骨细胞和成骨细胞作用的偶联有关。
