Lee D H, Tamura T, Chung C H, Tanaka K, Ichihara A
Institute for Enzyme Research, University of Tokushima, Japan.
Biochem Int. 1991 Mar;23(4):689-96.
The YC7-alpha gene encoding a subunit of yeast proteasomes named PRS2 has been isolated and sequenced. Southern blot analysis after electrophoretic separation of yeast chromosomal DNAs showed that the PRS2 gene is located in chromosome VII, unlike the PRS1 gene for subunit YC1, is located on chromosome XV. Surprisingly, the overall structure of the PRS2 gene was found to be identical to that of the suppressor scl1+ gene restoring the SCL1-1 mutation that suppresses crl3 cycloheximide-resistant, temperature-sensitive lethality. The identity of these two genes indicates that the suppressive role of the scl1+ gene product on crl3 mutation could be attributed to proteasomal function.
编码酵母蛋白酶体一个名为PRS2亚基的YC7-α基因已被分离并测序。对酵母染色体DNA进行电泳分离后的Southern印迹分析表明，PRS2基因位于第七条染色体上，这与编码亚基YC1的PRS1基因位于第十五条染色体上不同。令人惊讶的是，发现PRS2基因的整体结构与抑制SCL1-1突变的抑制基因scl1+的结构相同，该突变可抑制crl3环己酰亚胺抗性、温度敏感致死性。这两个基因的一致性表明，scl1+基因产物对crl3突变的抑制作用可能归因于蛋白酶体功能。
