Akioka H, Forsberg N E, Ishida N, Okumura K, Nogami M, Taguchi H, Noda C, Tanaka K
Institute for Enzyme Research, Tokushima University, Japan.
Biochem Biophys Res Commun. 1995 Feb 6;207(1):318-23. doi: 10.1006/bbrc.1995.1190.
For study of the molecular basis of regulation of proteasome gene expression, we isolated the gene encoding the alpha-type HC8 subunit of the human proteasome. About 2.3 kb of the 5' flanking region of this gene was tested for promoter function by chloramphenicol acetyltransferase assay. This analysis revealed that CAAT and TATA boxes, but not a GC box, are essential for its promoter activity. These results differed from previous findings that the genes for the alpha-type HC3 and beta-type HC5 subunits of the human proteasome have a TATA-less promoter and that two or three GC boxes function as the promoter sequences (Tamura, T. et al. (1994) J. Mol. Biol. 244, 1117-1124). We mapped the HC8 gene at q23 on human chromosome 14, which differs from the chromosomal locations of nine other proteasomal subunit genes mapped so far.
为了研究蛋白酶体基因表达调控的分子基础，我们分离出了编码人蛋白酶体α型HC8亚基的基因。通过氯霉素乙酰转移酶测定法，对该基因5'侧翼区域约2.3 kb的片段进行了启动子功能测试。该分析表明，CAAT盒和TATA盒对其启动子活性至关重要，而GC盒并非如此。这些结果与之前的发现不同，之前发现人蛋白酶体α型HC3和β型HC5亚基的基因具有无TATA启动子，且两到三个GC盒作为启动子序列发挥作用（Tamura, T.等人（1994年）《分子生物学杂志》244卷，第1117 - 1124页）。我们将HC8基因定位在人类14号染色体的q23上，这与目前已定位的其他九个蛋白酶体亚基基因的染色体位置不同。
