Bodnaryk R P
Can J Biochem. 1976 Nov;54(11):957-62. doi: 10.1139/o76-138.
Within 1 h after topical application of a convulsive dose (4 mug per fly, 47 mg/kg) of p,p'-dichlorodiphenyltrichloroethane (DDT) to the adult male of Sarcophaga bullata Parker, guanosine 3',5'-cyclic monophosphate (cyclic GMP) levels rose by 71.5% (P less than 0.05) in the head, 159.5% (P less than 0.01) in the thorax, and 23.4% (P greater than 0.05) in the abdomen compared to controls. Adenosine 3',5'-cyclic monophosphate (cyclic AMP) levels were not significantly affected by the DDT treatment. A convulsive dose (100 mug per larva, 250 mg/kg) of DDT applied to larvae of Mamestra configurata Wlk. caused the whole body level of cyclic GMP to rise by 81.6% (P less than 0.01) after 1 h, and by 95.9% (P less than 0.01) after 3 h. Levels of cyclic AMP were not affected. A hypothesis is advanced suggesting that an abnormally high rate of discharge of acetylcholine (and in the later stages of poisoning, its actual accumulation) at central cholinergic synapses causes cyclic GMP levels to rise, perhaps in post-synaptic cells. The elevated cyclic GMP-cyclic AMP ratio found in DDT-poisoned insects may be of fundamental importance in the complex sequence of events leading to tremor, hyperexcitability, paralysis, and death.
对红头丽蝇（Sarcophaga bullata Parker）成年雄蝇局部施用惊厥剂量（每只苍蝇4微克，47毫克/千克）的对，对'-二氯二苯三氯乙烷（DDT）后1小时内，与对照组相比，头部的环磷酸鸟苷（cGMP）水平升高了71.5%（P<0.05），胸部升高了159.5%（P<0.01），腹部升高了23.4%（P>0.05）。DDT处理对环磷酸腺苷（cAMP）水平没有显著影响。对甘蓝夜蛾（Mamestra configurata Wlk.）幼虫施用惊厥剂量（每只幼虫100微克，250毫克/千克）的DDT后，1小时后全身cGMP水平升高了81.6%（P<0.01），3小时后升高了95.9%（P<0.01）。cAMP水平未受影响。本文提出一个假说，即中枢胆碱能突触处乙酰胆碱异常高的释放速率（以及中毒后期其实际积累）导致cGMP水平升高，可能是在突触后细胞中。在DDT中毒昆虫中发现的升高的cGMP-cAMP比值，在导致震颤、过度兴奋、麻痹和死亡的复杂事件序列中可能具有根本重要性。
