混合溶剂中的低温酶学，无共溶剂对酶比活性的影响。
Cryoenzymology in mixed solvents without cosolvent effects on enzyme specific activity.
作者信息
Douzou P, Balny C
出版信息
Proc Natl Acad Sci U S A. 1977 Jun;74(6):2297-300. doi: 10.1073/pnas.74.6.2297.
Abstract
Water-soluble polyelectrolytes in interaction with proteins are described. These polyelectrolytes make it possible to investigate enzyme-catalyzed reactions in cooled mixed solvents without the usual effects of their organic solvent component on enzyme specific activity. The applicability of techniques developed is illustrated by results obtained on several systems. The possibility of an electrostatic "sorting out" of solvents and its potentialities in cryoenzymology are discussed.
摘要
本文描述了与蛋白质相互作用的水溶性聚电解质。这些聚电解质使得在冷却的混合溶剂中研究酶催化反应成为可能，而不会受到有机溶剂成分对酶比活性的常规影响。通过在几个系统上获得的结果说明了所开发技术的适用性。讨论了溶剂静电“分离”的可能性及其在低温酶学中的潜力。
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