Douzou P, Balny C, Franks F
Biochimie. 1978;60(2):151-8. doi: 10.1016/s0300-9084(78)80748-2.
A water in oil emulsion technique is proposed to investigate enzyme catalyzed reactions at sub-zero temperatures in the supercooled liquid state to avoid some reversible effects of the usual cosolvents on kinetics. Some results are listed: potentialities and technical problems of the procedure are discussed.