Velasco F, Velasco M, Maldonado H, Estrada-Villanueva F
Epilepsia. 1976 Dec;17(4):461-75. doi: 10.1111/j.1528-1157.1976.tb04458.x.
Nonspecific cortical, thalamic, mesencephalic, and pontine multiple unit activities (MUA) and changes in EEG and MUS of the sciatic nerve after threshold pentylenetetrazol activation were studied in three groups of animals in which neuronal connections were interrupted at three different levels of the central nervous system: spinal, mesencephalic, and prethalamic. Maximal increments of nonspecific MUA and maximal increments and maximal decrements of sciatic MUA after pentylenetetrazol from each group of lesioned animals were statistically compared with tose observed in intact animals. 1. Pentylenetetrazol threshold for producing cortical tonic-clonic EEG discharges was increased in animals with nesencephaic and prethalamic lesions but was not modified in animals with spinal transection. 2. Cortical MUA maximal increment was significantly decreased in mesencephalic and prethalamic lesioned animals, whereas thalamic MUA maximal increment was significantly decreased in mesencephalic and significantly increased in prethalamic lesioned animals. Pontine MUA maximal increment was significantly increased in spinal, mesencephalic, and prethalamic lesioned animals, and mesencephalic M-A maximal increment was not significantly modified in either prethalamic lesioned or in spinal transected animals. 3. Sciatic MUA maximal increment and maximal decrement were significatly decreased in spinal transected animals, whereas only maximal increment was significantly decreased in mesencephalic and only maximal decrement was significantly decreased in prethalamic lesioned animals. These results based on lesion experiments permit us to infer than under normal cinditions the development of generalized seizures induced by threshold pentylenetetrazol injection is highly dependent upon the neuronal interactions between nonspecific structures at different levels of the central nervous system. The possible nature of these neuronal interactions in the intact animals is discussed.
在三组动物中研究了阈下戊四氮激活后非特异性皮质、丘脑、中脑和脑桥的多单位活动（MUA）以及坐骨神经脑电图（EEG）和肌肉动作电位（MUS）的变化。这三组动物的神经元连接在中枢神经系统的三个不同水平被中断：脊髓、中脑和丘脑前。将每组损伤动物在戊四氮作用后非特异性MUA的最大增量以及坐骨神经MUA的最大增量和最大减量与完整动物中观察到的进行统计学比较。1. 中脑和丘脑前损伤的动物产生皮质强直-阵挛性EEG放电的戊四氮阈值升高，但脊髓横断的动物中该阈值未改变。2. 中脑和丘脑前损伤的动物皮质MUA最大增量显著降低，而丘脑MUA最大增量在中脑损伤动物中显著降低，在丘脑前损伤动物中显著增加。脑桥MUA最大增量在脊髓、中脑和丘脑前损伤的动物中显著增加，并且中脑M-A最大增量在丘脑前损伤动物或脊髓横断动物中均未显著改变。3. 脊髓横断的动物坐骨神经MUA最大增量和最大减量均显著降低，而在中脑损伤动物中只有最大增量显著降低，在丘脑前损伤动物中只有最大减量显著降低。基于损伤实验的这些结果使我们能够推断，在正常情况下，阈下戊四氮注射诱发的全身性癫痫发作的发展高度依赖于中枢神经系统不同水平非特异性结构之间的神经元相互作用。讨论了完整动物中这些神经元相互作用的可能性质。
