Rowley S, Carpenter R, Newbold K M, Gearty J, Keighley M R, Donovan I A, Neoptolemos J P
Academic Department of Surgery, Dudley Road Hospital, Birmingham, UK.
Eur J Surg Oncol. 1991 Aug;17(4):370-8.
The expression of the c-myc protein product (p62 c-myc) and deoxyribonucleic acid (DNA) ploidy status was determined by a flow cytometric technique in 83 patients with colorectal cancer followed up for a median of 30 months (range 6-60 months). The OM-11-906 antibody, used to detect p62 c-myc, revealed a 62 kDa and 45 kDa band on Western blots in tumours. Correlation of quantitative dot blotting of tumour mRNA to flow cytometric p62 c-myc expression was good (r = 0.87, P less than 0.01). Levels of p62 c-myc varied in colorectal cancer and low levels (less than 20 fluorescein units) correlated with improved survival (log rank chi 2 = 4.69, df = 1, P = 0.03), and this was a better prognostic index than DNA ploidy (log rank analysis chi 2 = 2.38, df = 1, P less than 0.1). Although expression of the c-myc gene was found, using the OM-11-906 antibody, to be a prognostic feature in colorectal cancer, these and other results need to be interpreted with caution given the presence of two protein bands by Western blotting.
采用流式细胞术对83例结肠癌患者进行c-myc蛋白产物（p62 c-myc）表达及脱氧核糖核酸（DNA）倍体状态检测，随访时间中位数为30个月（范围6 - 60个月）。用于检测p62 c-myc的OM-11-906抗体在肿瘤组织的蛋白质免疫印迹法中显示出一条62 kDa和一条45 kDa的条带。肿瘤mRNA定量斑点印迹法与流式细胞术检测的p62 c-myc表达具有良好的相关性（r = 0.87，P < 0.01）。p62 c-myc水平在结肠癌中存在差异，低水平（< 20荧光素单位）与生存率提高相关（对数秩检验χ2 = 4.69，自由度 = 1，P = 0.03），并且这是一个比DNA倍体更好的预后指标（对数秩分析χ2 = 2.38，自由度 = 1，P < 0.1）。尽管使用OM-11-906抗体发现c-myc基因的表达是结肠癌的一个预后特征，但鉴于蛋白质免疫印迹法中存在两条蛋白条带，这些结果以及其他结果需要谨慎解读。
