Hasková V, Rozprimová L, Svobodová J
Institute for Clinical and Experimental Medicine, Praha.
Folia Biol (Praha). 1991;37(2):134-9.
We isolated and characterized a non-immunoglobulin fraction from calf thymuses which was found very active in binding [3H]labelled cyclosporine (CsA). It contains the natural, intracellular receptor cyclophilin, specifically binding to the active site of the CsA molecule, which may also play a part in the immunosuppression of human immunocompetent cells. This material with a high selectivity could be used, instead of specific monoclonal or polyclonal antibodies, to determine CsA levels in patient's blood.
我们从小牛胸腺中分离并鉴定出一种非免疫球蛋白组分，发现它在结合[3H]标记的环孢素（CsA）方面非常活跃。它含有天然的细胞内受体亲环蛋白，能特异性结合CsA分子的活性位点，这在人类免疫活性细胞的免疫抑制中可能也起作用。这种具有高选择性的物质可替代特异性单克隆或多克隆抗体，用于测定患者血液中的CsA水平。
