van Tol E A, Verspaget H W, Peña A S, Jansen J B, Aparicio-Pagés M N, Lamers C B
Department of Gastroenterology and Hepatology, University Hospital, Leiden, The Netherlands.
Immunol Invest. 1991 Jun;20(3):257-67. doi: 10.3109/08820139109026229.
In the present study the effect of vasoactive intestinal peptide (VIP), peptide histidine-methionine (PHM), and secretin on spontaneous cell mediated cytotoxicity of peripheral blood mononuclear cells against tumour target cells was evaluated. VIP stimulated cytotoxicity against CaCo-2 human colon cancer cells, whereas less effect was seen against K-562 erythroleukemia cells. Depletion of CD16+ natural killer cells almost completely abolished cytotoxicity and subsequent VIP incubation did not change residual activity. In contrast to PHM, which hardly influenced cytotoxicity, secretin was found to be more effective especially against K-562 target cells. These observations suggest a modulating role for the neuropeptide VIP in the cellular immune response against tumour cells, especially from the colon, resulting in increased activity of CD16+ natural killer cells. Secretin, seems to be less potent in modulating cellular cytotoxicity. These findings support the concept that gastrointestinal peptides can play a role in the regulation of cellular cytotoxicity against tumor cells.
在本研究中，评估了血管活性肠肽（VIP）、肽组氨酸甲硫氨酸（PHM）和促胰液素对外周血单个核细胞对肿瘤靶细胞的自发细胞介导细胞毒性的影响。VIP刺激了对CaCo-2人结肠癌细胞的细胞毒性，而对K-562红白血病细胞的影响较小。CD16+自然杀伤细胞的耗竭几乎完全消除了细胞毒性，随后的VIP孵育并未改变残余活性。与几乎不影响细胞毒性的PHM相反，发现促胰液素更有效，尤其是对K-562靶细胞。这些观察结果表明神经肽VIP在针对肿瘤细胞，尤其是结肠肿瘤细胞的细胞免疫反应中具有调节作用，导致CD16+自然杀伤细胞活性增加。促胰液素在调节细胞毒性方面似乎效力较小。这些发现支持了胃肠肽可在调节针对肿瘤细胞的细胞毒性中发挥作用这一概念。
