采用邻苯三酚红-钼络合物分光光度检测的高效液相色谱法测定尿液中的蛋白质。
Determination of proteins in urine by high-performance liquid chromatography with spectrophotometric detection using a pyrogallol red-molybdate complex.
作者信息
Mashige F, Ohkubo A
机构信息
Department of Laboratory Medicine, Faculty of Medicine, University of Tokyo, Japan.
出版信息
J Chromatogr. 1991 Apr 19;565(1-2):173-81. doi: 10.1016/0378-4347(91)80381-l.
A high-performance liquid chromatographic method with spectrophotometric detection was developed for the determination of proteins in urine. The proteins were separated on an anion-exchange column and eluted with a Tris-HCl buffer with a gradient of sodium chloride concentration and pH. The separated proteins were mixed with a pyrogallol red-molybdate complex reagent and determined spectrophotometrically. Urinary proteins were well separated without desalting the urine. The reproducibility was satisfactory.
建立了一种采用分光光度检测的高效液相色谱法测定尿液中的蛋白质。蛋白质在阴离子交换柱上进行分离，并用含氯化钠浓度和pH梯度的Tris-HCl缓冲液洗脱。分离出的蛋白质与邻苯三酚红-钼酸盐复合试剂混合，然后进行分光光度测定。尿液蛋白质无需脱盐即可得到良好分离。重现性令人满意。
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