Kinahan I M, Smyth M R
School of Chemical Sciences, Dublin City University, Ireland.
J Chromatogr. 1991 Apr 19;565(1-2):297-307. doi: 10.1016/0378-4347(91)80391-o.
Polyethylene glycols (PEGs) are non-ionic, water-soluble synthetic polymers which have been widely used for many applications. Since they are of very low toxicity and are readily excreted in urine, PEGs in the molecular weight range 400-6000 have been used extensively in the study of intestinal physiology in man. A high-performance liquid chromatographic (HPLC) method has been developed for the determination of PEG 600 in human urine, which includes a pre-column derivatisation step. The dibenzoate derivatives of PEG 600 can be quantitatively prepared, and this, coupled with ultraviolet detection at 230 nm, has greatly improved the limit of detection for the determination of PEGs by HPLC. A suitable extraction procedure has also been developed which enabled PEG levels in urine to be monitored with much greater sensitivity than any previously reported method.
聚乙二醇（PEGs）是一种非离子型水溶性合成聚合物，已广泛应用于许多领域。由于它们毒性极低且易于经尿液排出，分子量范围在400 - 6000的聚乙二醇已被广泛用于人体肠道生理学研究。已开发出一种高效液相色谱（HPLC）法用于测定人尿液中的聚乙二醇600，该方法包括柱前衍生步骤。聚乙二醇600的二苯甲酸酯衍生物能够定量制备，再结合在230 nm处的紫外检测，极大地提高了通过高效液相色谱法测定聚乙二醇的检测限。还开发了一种合适的萃取程序，该程序能够比以往任何报道的方法以更高的灵敏度监测尿液中的聚乙二醇水平。
