Young G O, Ruttenberg D, Wright J P
Department of Medicine, University of Cape Town, South Africa.
Clin Chem. 1990 Oct;36(10):1800-2.
We describe a new "high-performance" liquid chromatographic (HPLC) method for estimating polyethylene glycol 400 (PEG 400) in urine. Direct injection of diluted urine samples onto the column eliminates the necessity for lengthy preparative steps, resulting in considerable saving of both time and chemicals. High analytical recoveries (94-102%) and a rapid rate of analysis are achieved. Results vary linearly with concentrations from 0.2 to 1.6 g/L and the six major components can be individually quantified precisely at all concentrations in this range. This method shows better sample recovery, sensitivity, and reproducibility than does a previously described method. The increased sensitivity should allow the use of lower oral doses of polyethylene glycol 400 in future studies of intestinal permeability.
我们描述了一种用于估算尿液中聚乙二醇400（PEG 400）的新型“高性能”液相色谱（HPLC）方法。将稀释后的尿液样本直接注入色谱柱，无需冗长的制备步骤，从而大量节省了时间和化学试剂。该方法具有较高的分析回收率（94 - 102%）且分析速度快。结果在0.2至1.6 g/L的浓度范围内呈线性变化，在此范围内的所有浓度下，六种主要成分均可精确地单独定量。与先前描述的方法相比，该方法具有更好的样品回收率、灵敏度和重现性。更高的灵敏度应有助于在未来肠道通透性研究中使用更低口服剂量的聚乙二醇400。
