Perkhofer Susanne, Kehrel Beate E, Dierich Manfred P, Donnelly J Peter, Nussbaumer Walter, Hofmann Johann, von Eiff Christof, Lass-Flörl Cornelia
Department of Hygiene, Innsbruck Medical University, Austria.
J Infect Dis. 2008 Oct 15;198(8):1243-6. doi: 10.1086/591458.
Using laser scanning microscopy, we investigated whether platelets are capable of internalizing Aspergillus conidia and examined Aspergillus-platelet adherence. The influence of platelets on fungal growth was evaluated by assessing galactomannan (GM) release, hyphal elongation, and colony size. A secretion assay with [(3)H]-serotonin (5-hydroxytryptamine [5-HT]) was performed. Exposure to platelets resulted in significantly decreased GM release (p<.05), hyphal elongation (p<.001), colony size, pigmentation, and 5-HT release ( p<.05). A lack of antifungal effects was observed with the microfilament inhibitor cytochalasin D. Platelets attenuate the virulence of Aspergillus species in vitro on the basis of granule-dependent effects.
我们使用激光扫描显微镜研究了血小板是否能够内化曲霉分生孢子,并检测了曲霉与血小板的黏附情况。通过评估半乳甘露聚糖(GM)释放、菌丝伸长和菌落大小来评价血小板对真菌生长的影响。进行了用[³H] - 血清素(5 - 羟色胺[5 - HT])的分泌试验。暴露于血小板导致GM释放显著减少(p <.05)、菌丝伸长显著减少(p <.001)、菌落大小、色素沉着和5 - HT释放显著减少(p <.05)。使用微丝抑制剂细胞松弛素D未观察到抗真菌作用。基于颗粒依赖性效应,血小板在体外可减弱曲霉属真菌的毒力。