Li D, Wen X, Ghali L, Al-Shalabi F M, Docherty S M, Purkis P, Iles R K
Biomedical Science, Department of Natural Sciences, School of Health and Social Science, Middlesex University, Enfield, UK.
Histopathology. 2008 Aug;53(2):147-55. doi: 10.1111/j.1365-2559.2008.03082.x.
To investigate the correlation of beta-subunit of human chorionic gonadotrophin (hCG beta) expression by cervical carcinomas with measures of tumour apoptosis.
Eighty-nine cervical carcinoma patients' samples were subject to hCG beta immunohistochemistry and scored with respect to intensity of immunopositivity and percentage of positive cells. Apoptosis was evaluated by three independent parameters: morphological characteristics [haematoxylin and eosin (H&E)], terminal deoxynucleotidyl transferase biotin-dUTP nick end labelling (TUNEL) and poly (ADP-ribose) polymerase (PARP) immunopositivity. Of the 12 adenocarcinomas, only one (8%) was hCG beta+. However, 87% (61/70) of the squamous cell and 100% (7/7) of adenosquamous cell carcinomas were hCG beta+. hCG beta reactivity and intensity was predominantly confined to peripheral tumour cells at the stromal-epithelial interface. Correlation analysis showed that H&E and PARP apoptotic immunopositivity negatively correlated with hCG beta expression (P < 0.001 and P = 0.028 respectively), whereas TUNEL did not (P = 0.12). However, immunopositivity for apoptotic cells by TUNEL was significantly less in tumours where hCG beta expression was greater (scoring >or= 6) and vice versa. hCG beta immunopositivity was also observed in newly formed blood vessels, as well as tumour cells within lymphatic vessels. When tumour vascularization was taken into account, samples with noted vascularization positively correlated with hCG beta scoring.
hCG beta expression correlates with reduced tumour cell apoptosis and may be involved in tumour vascularization and dissemination.
研究宫颈癌中人绒毛膜促性腺激素β亚基（hCGβ）表达与肿瘤凋亡指标的相关性。
对89例宫颈癌患者的样本进行hCGβ免疫组织化学检测，并根据免疫阳性强度和阳性细胞百分比进行评分。通过三个独立参数评估凋亡情况：形态学特征[苏木精和伊红（H&E）染色]、末端脱氧核苷酸转移酶生物素-dUTP缺口末端标记法（TUNEL）和聚（ADP-核糖）聚合酶（PARP）免疫阳性。在12例腺癌中，只有1例（8%）为hCGβ阳性。然而，鳞状细胞癌中有87%（61/70）和腺鳞癌中有100%（7/7）为hCGβ阳性。hCGβ反应性和强度主要局限于基质-上皮界面的外周肿瘤细胞。相关性分析显示，H&E和PARP凋亡免疫阳性与hCGβ表达呈负相关（分别为P<0.001和P = 0.028），而TUNEL则无相关性（P = 0.12）。然而，在hCGβ表达较高（评分≥6）的肿瘤中，TUNEL检测到的凋亡细胞免疫阳性明显较少，反之亦然。在新生血管以及淋巴管内的肿瘤细胞中也观察到hCGβ免疫阳性。当考虑肿瘤血管生成时，有明显血管生成的样本与hCGβ评分呈正相关。
hCGβ表达与肿瘤细胞凋亡减少相关，可能参与肿瘤血管生成和扩散。
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