大鼠孕期子宫引流淋巴结中收获的淋巴细胞的淋巴细胞增殖反应动力学。
Kinetics of lymphoproliferative responses of lymphocytes harvested from the uterine draining lymph nodes during pregnancy in rats.
作者信息
Kapovic M, Rukavina D
机构信息
Department of Physiology and Immunology, Faculty of Medicine, University of Rijeka, Yugoslavia.
出版信息
J Reprod Immunol. 1991 May;20(1):93-101. doi: 10.1016/0165-0378(91)90026-m.
Paraaortic lymph node (PALN) cells were harvested from virgin and pregnant rats bearing syngeneic or allogeneic fetuses at all stages of pregnancy including the pre-implantation period. The specific and non-specific alloreactivity of these cells was analyzed in mixed lymphocyte reactivity (MLR) against mitomycin-C treated-paternal strain or unrelated cells. Mitogen stimulation of the cell utilized PHA, Con-A and PWM. Cells bearing T cell markers were labeled in an indirect assay using the monoclonal antibodies W3/25 and MRC OX 8. Specific alloreactivity is strongly suppressed in the pre-implantation and implantation stages of pregnancy. Specific and non-specific alloreactivities were enhanced at mid-pregnancy and normalized by the end of pregnancy. Reactivity to polyclonal mitogens is enhanced at mid-pregnancy, and the CD4+/CD8+ ratio is very low during all phases of pregnancy.
从怀孕各阶段（包括植入前期）怀有同基因或异基因胎儿的未孕大鼠和怀孕大鼠中采集主动脉旁淋巴结（PALN）细胞。在针对丝裂霉素C处理的父本菌株或无关细胞的混合淋巴细胞反应（MLR）中分析这些细胞的特异性和非特异性同种异体反应性。细胞的丝裂原刺激采用PHA、Con-A和PWM。使用单克隆抗体W3/25和MRC OX 8在间接测定中标记带有T细胞标志物的细胞。在怀孕的植入前期和植入期，特异性同种异体反应受到强烈抑制。在怀孕中期，特异性和非特异性同种异体反应增强，并在怀孕末期恢复正常。在怀孕中期，对多克隆丝裂原的反应增强，并且在怀孕的所有阶段CD4+/CD8+比率都非常低。
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