Satterfield M Carey, Hayashi Kanako, Song Gwonhwa, Black Sarah G, Bazer Fuller W, Spencer Thomas E
Center for Animal Biotechnology and Genomics, Department of Animal Science, Texas A&M University, College Station, Texas 77843-2471, USA.
Biol Reprod. 2008 Dec;79(6):1226-36. doi: 10.1095/biolreprod.108.071787. Epub 2008 Aug 27.
Progesterone (P4) is unequivocally required to maintain a uterine environment conducive to pregnancy. This study investigated the effects of P4 treatment on expression of selected growth factors (fibroblast growth factor 7 [FGF7], FGF10, hepatocyte growth factor [HGF], and insulin-like growth factors [IGF1 and IGF2]), their receptors (MET, FGFR2(IIIB), and IGF1R), and IGF binding proteins (IGFBPs) in the ovine uterus. Ewes received daily injections of corn oil vehicle (CO) or 25 mg of P4 in vehicle from 36 h after mating (Day 0) to hysterectomy on Day 9 or Day 12. Another group received P4 to Day 8 and 75 mg of mifepristone (RU486, a P4 receptor antagonist) from Day 8 through Day 12. Endometrial FGF10 mRNA levels increased between Day 9 and Day 12 and in response to P4 on Day 9 in CO-treated ewes, which had larger blastocysts, and were substantially reduced in P4+RU486-treated ewes, which had no blastocysts on Day 12. Endometrial FGF7 or HGF mRNA levels were not affected by day or reduced by RU486 treatment, but MET mRNA levels were higher in P4-treated ewes on Day 9 and Day 12. Levels of IGF1, IGF2, and IGF1R mRNA in the endometria were not affected by early P4 treatment. Although stromal IGFBPs were unaffected by P4, levels of IGFBP1 and IGFBP3 mRNA in uterine luminal epithelia were increased substantially between Day 9 and Day 12 of pregnancy in CO-treated ewes and on Day 9 in early P4-treated ewes. Therefore, FGF10, MET, IGFBP1, and IGFBP3 are P4-regulated factors within the endometrium of the ovine uterus that have potential effects on endometrial function and peri-implantation blastocyst growth and development.
孕酮（P4）对于维持有利于妊娠的子宫环境是绝对必需的。本研究调查了P4处理对绵羊子宫中所选生长因子（成纤维细胞生长因子7 [FGF7]、FGF10、肝细胞生长因子 [HGF] 和胰岛素样生长因子 [IGF1和IGF2]）、它们的受体（MET、FGFR2（IIIB）和IGF1R）以及IGF结合蛋白（IGFBPs）表达的影响。母羊在交配后36小时（第0天）至第9天或第12天子宫切除期间，每天注射玉米油载体（CO）或载体中含25 mg P4。另一组在第8天前接受P4注射，从第8天至第12天接受75 mg米非司酮（RU486，一种P4受体拮抗剂）。在CO处理的母羊中，子宫内膜FGF10 mRNA水平在第9天至第12天之间升高，并且在第9天对P4有反应，这些母羊的囊胚较大；而在P4 + RU486处理的母羊中，子宫内膜FGF10 mRNA水平大幅降低，这些母羊在第12天没有囊胚。子宫内膜FGF7或HGF mRNA水平不受天数影响，也未因RU486处理而降低，但在第9天和第12天，P4处理的母羊中MET mRNA水平较高。早期P4处理对子宫内膜中IGF1、IGF2和IGF1R mRNA水平没有影响。虽然基质IGFBPs不受P4影响，但在CO处理的母羊妊娠第9天至第12天期间以及早期P4处理的母羊第9天时，子宫腔上皮中IGFBP1和IGFBP3 mRNA水平大幅升高。因此，FGF10、MET、IGFBP1和IGFBP3是绵羊子宫内膜中受P4调节的因子，对子宫内膜功能以及植入前囊胚的生长和发育具有潜在影响。
