Postlethwait J H
Institute of Neuroscience, University of Oregon, Eugene, Oreg., USA.
Genome Dyn. 2006;2:183-197. doi: 10.1159/000095104.
Zebrafish is one of several important teleost models for understanding principles of vertebrate developmental, molecular, organismal, genetic, evolutionary, and genomic biology. Efficient investigation of the molecular genetic basis of induced mutations depends on knowledge of the zebrafish genome. Principles of zebrafish genomic analysis, including gene mapping, ortholog identification, conservation of syntenies, genome duplication, and evolution of duplicate gene function are discussed here using as a case study the zebrafish msxa, msxb, msxc, msxd, and msxe genes, which together constitute zebrafish orthologs of tetrapod Msx1, Msx2, and Msx3. Genomic analysis suggests orthologs for this difficult to understand group of paralogs.
斑马鱼是用于理解脊椎动物发育、分子、机体、遗传、进化和基因组生物学原理的几种重要硬骨鱼模型之一。对诱导突变的分子遗传基础进行有效研究依赖于对斑马鱼基因组的了解。本文以斑马鱼msxa、msxb、msxc、msxd和msxe基因作为案例研究，讨论了斑马鱼基因组分析的原理，包括基因定位、直系同源物鉴定、同线性保守、基因组复制以及重复基因功能的进化，这些基因共同构成了四足动物Msx1、Msx2和Msx3的斑马鱼直系同源物。基因组分析表明了这一难以理解的旁系同源物群体的直系同源物。
