Gallard Anthony, Panis Bart, Dorion Nöelle, Swennen Rony, Grapin Agnès
UMR GenHort, INRA, INH, UA, Institut National dHorticulture, Angers Cedex 01, France.
Cryo Letters. 2008 May-Jun;29(3):243-51.
The droplet-vitrification method was adapted to Pelargonium apices by optimizing the duration of the loading solution (LS) as well as the plant vitrification solution 2 (PVS2). The excised apices were dehydrated in two steps (20 min in LS and 15 min in PVS2) and then immersed directly in liquid nitrogen (LN). After thawing and unloading in the recovery solution at room temperature for 15 min, apices were plated onto semi-solid Murashige and Skoog medium. This simple protocol without any pretreatment was successfully applied to eight cultivars with a survival level ranging between 55.6 - 96.2 percent and a regrowth level between 9.1 and 70.6 percent. These results prove the feasibility of the long-term storage of Pelargonium germplasm through cryopreservation.
通过优化装载溶液（LS）以及植物玻璃化溶液2（PVS2）的处理时间，将液滴玻璃化法应用于天竺葵茎尖。切下的茎尖分两步脱水（在LS中处理20分钟，在PVS2中处理15分钟），然后直接浸入液氮（LN）中。在室温下于恢复溶液中解冻并卸载15分钟后，将茎尖接种到半固体Murashige和Skoog培养基上。这个无需任何预处理的简单方案成功应用于8个品种，存活率在55.6%至96.2%之间，再生率在9.1%至70.6%之间。这些结果证明了通过冷冻保存长期储存天竺葵种质的可行性。
