Park Hee-Jin, Kim Hyun-Joong, Park Si-Hong, Shin Eun-Gyeong, Kim Jae-Hwan, Kim Hae-Yeong
Institute of Life Sciences and Resources, and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Korea.
J Microbiol Biotechnol. 2008 Aug;18(8):1453-8.
For quantitative PCR assay of Salmonella enterica serovar Typhimurium in food samples, a real-time PCR method was developed, based on DNA genome equivalent. Specific primers and probe designed based on the STM4497 gene of S. Typhimurium LT2 showed the specificity to S. Typhimurium. Threshold cycle (Ct) values of real-time PCR were obtained from a quantitative standard curve with genomic DNA of Salmonella Typhimurium. In addition, the recovery of S. Typhimurium inoculated artificially to chicken samples with 4.5 x 10(5) to 4.5 CFU/ml was evaluated by using real-time PCR and plate-count methods. Result showed that the number of cells calculated from the real-time PCR method had good correlation with that of the plate-count method. This real-time PCR method could be applicable to the detection and quantification of S. Typhimurium in food samples.
为了对食品样本中的肠炎沙门氏菌鼠伤寒血清型进行定量PCR检测，基于DNA基因组当量开发了一种实时PCR方法。根据鼠伤寒沙门氏菌LT2的STM4497基因设计的特异性引物和探针显示出对鼠伤寒沙门氏菌的特异性。实时PCR的阈值循环（Ct）值来自鼠伤寒沙门氏菌基因组DNA的定量标准曲线。此外，通过实时PCR和平板计数法评估了人工接种到鸡肉样本中浓度为4.5×10⁵至4.5 CFU/ml的鼠伤寒沙门氏菌的回收率。结果表明，通过实时PCR方法计算的细胞数量与平板计数法的结果具有良好的相关性。这种实时PCR方法可用于食品样本中鼠伤寒沙门氏菌的检测和定量。
