Xu Xiaoping, Kong Fanrong, Cheng Xun, Yan Bing, Du Xin, Gai Junhui, Ai Hui, Shi Lei, Iredell Jon
Department of Laboratory Medicine, Shenzhen Second People's Hospital, Shenzhen 518035, China.
Int J Antimicrob Agents. 2008 Nov;32(5):441-5. doi: 10.1016/j.ijantimicag.2008.05.014. Epub 2008 Aug 30.
The epidemiology of emerging antibiotic resistance genes in Asia is inadequately defined and studies within the major pools of transmissible genes such as integron gene cassettes are important. One hundred and twenty-two non-repetitive Acinetobacter spp. isolates were obtained from inpatients of a major hospital in South China. Fifty-three of these isolates contained class 1 integrons, and there is evidence of horizontal gene transfer between unrelated clones. The common pool of gene cassettes was dominated by four cassette arrays: arr3-aacA4 (24 isolates of several unrelated strains); aacC1-orfP-orfQ-aadA1a (11 isolates, probably all the same strain); aacA4-catB8-aadA1 (2 isolates); and dfrVII (1 isolate). We developed a simple restriction fragment length polymorphism (RFLP)-based identification of these and other cassettes reported in China, using readily available enzymes, to facilitate further studies of this type.
亚洲新出现的抗生素耐药基因的流行病学情况尚未得到充分界定，对可传播基因的主要库（如整合子基因盒）进行研究很重要。从中国南方一家大型医院的住院患者中分离出122株非重复性不动杆菌属菌株。其中53株分离株含有1类整合子，并且有证据表明不相关克隆之间存在水平基因转移。基因盒的共同库由四种盒式阵列主导：arr3-aacA4（来自几种不相关菌株的24株分离株）；aacC1-orfP-orfQ-aadA1a（11株分离株，可能均为同一菌株）；aacA4-catB8-aadA1（2株分离株）；以及dfrVII（1株分离株）。我们利用容易获得的酶，开发了一种基于简单限制性片段长度多态性（RFLP）的方法来鉴定这些以及中国报道的其他盒式阵列，以促进此类进一步研究。
