Moriguchi Tomohisa, Sakai Hideaki, Suzuki Hideo, Shinozuka Kazuo
Graduate School of Engineering, Gunma University, Kiryu, Gunma, Japan.
Chem Pharm Bull (Tokyo). 2008 Sep;56(9):1259-63. doi: 10.1248/cpb.56.1259.
Novel phosphorothioate-modified oligodeoxynucleotides (S-ODNs) containing a deoxyuridine derivative bearing a spermine moiety at the C-5 position were synthesized. The study of the thermal stability and the thermodynamic stability showed that the modified S-ODNs have been able to form the stable duplexes with the complementary DNA. It was also found that the duplex composed of the modified S-ODN and its complementary RNA strand is the substrate for Escherichia coli RNase H, and the cleavage of the RNA strand by the enzyme was almost similar as in the case of the unmodified one.
合成了新型硫代磷酸酯修饰的寡脱氧核苷酸（S-ODN），其含有在C-5位带有精胺部分的脱氧尿苷衍生物。对热稳定性和热力学稳定性的研究表明，修饰后的S-ODN能够与互补DNA形成稳定的双链体。还发现由修饰后的S-ODN及其互补RNA链组成的双链体是大肠杆菌RNase H的底物，并且该酶对RNA链的切割与未修饰的情况几乎相似。
