Khan H A, Siddique M, Arshad M, Abubakar M, Akhtar M, Arshad M J, Ashraf M
Department of Microbiology, University of Agriculture, Faisalabad, Pakistan.
Trop Anim Health Prod. 2009 Apr;41(4):427-30. doi: 10.1007/s11250-008-9222-6. Epub 2008 Aug 30.
A total of 70 sheep and 330 goats were selected randomly. All the animals were kept under same housing and management conditions. Serum samples were collected from all the animals and tested for the presence of antibodies against Peste des petits ruminants (PPR) virus using competitive ELISA (cELISA). All the animals were found negative showing percentage inhibition (PI) values <50. The animals were vaccinated against PPR with Nig/75/1 strain vaccine of PPR Serum samples were collected from randomly selected 12 sheep and 30 goats at 10, 30 and 45 days post-vaccination. The samples were subjected to cELISA to determine the presence of antibodies against PPRV. The samples with PI >50 were considered as sero-positive. The sheep found positive at 10, 30 and 45 days post-vaccination were 1(8.3%), 7(58.3%) and 12(100%) respectively. In case of goats 3(10.0%), 29(96.6%) and 27(90.0%) animals gave positive results at 10, 30 and 45 days post-vaccination respectively. Mean PI values in sheep at 10, 30 and 45 days post-vaccination were recorded as 37, 65 and 91 respectively, whereas in goats these values were 43, 78 and 86 respectively.
随机挑选了70只绵羊和330只山羊。所有动物均饲养在相同的圈舍和管理条件下。采集所有动物的血清样本，使用竞争ELISA（cELISA）检测抗小反刍兽疫（PPR）病毒抗体的存在情况。所有动物检测结果均为阴性，抑制率（PI）值<50。用PPR Nig/75/1株疫苗对动物进行PPR疫苗接种。在接种疫苗后第10天、30天和45天，从随机挑选的12只绵羊和30只山羊中采集血清样本。对样本进行cELISA检测以确定抗PPRV抗体的存在情况。PI>50的样本被视为血清阳性。接种疫苗后第10天、30天和45天检测为阳性的绵羊分别为1只（8.3%）、7只（58.3%）和12只（100%）。山羊在接种疫苗后第10天、30天和45天检测为阳性的动物分别为3只（10.0%）、29只（96.6%）和27只（90.0%）。接种疫苗后第10天、30天和45天绵羊的平均PI值分别记录为37、65和91，而山羊的这些值分别为43、78和86。
