Yu Ying, Zhu Shiguo, Zhang Bicheng, Zhou Ming, Li Xiaoling, Li Guiyuan
Cancer Research Institute, Xiangya School of Medicine, Central South University, 410078, Changsha, China.
Sci China C Life Sci. 2002 Oct;45(5):546-52. doi: 10.1360/02yc9060.
BRD7 gene is low or undetectably expressed in nasopharyngeal carcinoma tissue (NPC) and can obviously suppress the growth of NPC cell line HNE1. In this study, the proteins that interacted with BRD7 coding protein were screened by yeast two-hybrid system. The complete reading frame of BRD7 gene was subcloned into pAS2 vector (BRD7-BD). Then the human embryo brain cDNA library was screened by BRD7-BD bait. Eleven positive clones were obtained from 4.8 x 10(6) transformed clones. By sequencing directly, 6 interacting proteins of BRD7 coding protein were isolated (Bromodomain-containing 3 protein (BRD3), Bromodomain-containing 2 protein (BRD2), I kappaB kinase-beta, KIAA1375 protein, interleukin 7 and adaptor-related protein complex 3 delta-1 subunit). These results suggest BRD7 protein might form heterogenous dimer or triplex polymer with BRD2 and/or BRD3 to participate in transcriptional regulation.
BRD7基因在鼻咽癌组织（NPC）中低表达或表达检测不到，并且能明显抑制鼻咽癌细胞系HNE1的生长。在本研究中，通过酵母双杂交系统筛选与BRD7编码蛋白相互作用的蛋白质。将BRD7基因的完整阅读框亚克隆到pAS2载体（BRD7-BD）中。然后用BRD7-BD诱饵筛选人胚胎脑cDNA文库。从4.8×10（6）个转化克隆中获得了11个阳性克隆。通过直接测序，分离出6种与BRD7编码蛋白相互作用的蛋白质（含溴结构域3蛋白（BRD3）、含溴结构域2蛋白（BRD2）、IκB激酶β、KIAA1375蛋白、白细胞介素7和衔接蛋白相关蛋白复合体3δ-1亚基）。这些结果表明BRD7蛋白可能与BRD2和/或BRD3形成异源二聚体或三聚体聚合物以参与转录调控。
