Zhou Ming, Xu Xiao-Jie, Zhou Hou-De, Liu Hua-Ying, He Jia-Jin, Li Xiao-Ling, Peng Cong, Xiong Wei, Fan Song-Qing, Lu Jian-Hong, Ouyang Jue, Shen Shou-Rong, Xiang Bo, Li Gui-Yuan
Cancer Research Institute, Central South University Xiang-Ya School of Medicine, Changsha, Hunan, 410078, China.
Mol Cell Biochem. 2006 Nov;292(1-2):205-12. doi: 10.1007/s11010-006-9233-4. Epub 2006 Jun 20.
BRD7 is a potential nuclear transcription regulation factor related to nasopharyngeal carcinoma (NPC). BRD2, a putative BRD7-interacting protein, has been screened from human fetal brain cDNA library by yeast two-hybrid system. This study was to further identify the interaction between BRD7 and BRD2 in mammalian cells, and to investigate the subcellular localization of BRD2, as well as the effect on the functions of cell biology. Both immunoprecipitation and subcellular colocalization were performed together to identify the interaction of BRD7 with full-length BRD2, as well as C-terminal truncated BRD2 or N-terminal truncated BRD2. GFP direct fluorescence and Hochest 33258 staining were used to investigate the cellular localization pattern of BRD2 and the roles in initiating cell apoptosis in COS7 and HNE1. The results showed that BRD7 could interact with BRD2 and the region from amino acid 430 to 798 of BRD2 was critical for the interaction of BRD2 with BRD7. BRD2 mainly localizes in nucleus in two distribution patterns, diffused and dotted, and BRD2 has distinct roles in initiating apoptosis, and the dotted distribution pattern of BRD2 in nucleus may be a morphologic marker of cell apoptosis.
BRD7是一种与鼻咽癌（NPC）相关的潜在核转录调控因子。BRD2是一种推测的与BRD7相互作用的蛋白，已通过酵母双杂交系统从人胎脑cDNA文库中筛选出来。本研究旨在进一步鉴定BRD7与BRD2在哺乳动物细胞中的相互作用，研究BRD2的亚细胞定位以及对细胞生物学功能的影响。通过免疫沉淀和亚细胞共定位共同鉴定BRD7与全长BRD2、C末端截短的BRD2或N末端截短的BRD2之间的相互作用。使用GFP直接荧光和Hochest 33258染色研究BRD2在COS7和HNE1细胞中的细胞定位模式以及启动细胞凋亡的作用。结果表明，BRD7可与BRD2相互作用，BRD2中430至798位氨基酸区域对于BRD2与BRD7的相互作用至关重要。BRD2主要以弥散和点状两种分布模式定位于细胞核，并且BRD2在启动细胞凋亡中具有不同作用，BRD2在细胞核中的点状分布模式可能是细胞凋亡的形态学标志。
