Lee C-H, Butt Y K-C, Wong M-S, Lo S C-L
Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong SAR.
Inflammopharmacology. 2008 Aug;16(4):188-94. doi: 10.1007/s10787-008-8010-2.
The lipid extract of Perna canaliculus (Lyprinol) has known anti-inflammatory effects. However, the only information on mechanisms is regulation of cytokine secretion. Therefore, we conducted a proteomic study exploring the effects of Lyprinol on protein expression in splenocytes collected from AIA rats. Splenocytes from AIA rats fed with Lyprinol had increased protein expression of malate dehydrogenase (MDH). Lyprinol also decreased the expressions of 5 other proteins: protein-o-mannosyl- transferase 2 (PMT-2), Tdrd 7, telethonin, dynactin 2 and protein disulfide isomerase (PDI or glucose-regulated protein (GRP)). Besides MDH, PMT- 2, titin-cap protein and protein disulfide isomerase (PDI) are known to be related to metabolism. However, it is currently unknown if Lyprinol administration decreases metabolic glucose in the body and alleviates symptoms of inflammation and arthritis. Further experiments are required to correlate levels of citric acid intermediates and glucose to the severity of inflammation and pain in AIA rats fed Lyprinol.
贻贝（Lyprinol）的脂质提取物具有抗炎作用。然而，关于其作用机制的唯一信息是对细胞因子分泌的调节。因此，我们进行了一项蛋白质组学研究，以探究Lyprinol对从佐剂性关节炎（AIA）大鼠采集的脾细胞中蛋白质表达的影响。喂食Lyprinol的AIA大鼠的脾细胞中，苹果酸脱氢酶（MDH）的蛋白质表达增加。Lyprinol还降低了其他5种蛋白质的表达：蛋白质O-甘露糖基转移酶2（PMT-2）、Tdrd 7、肌联蛋白、动力蛋白激活蛋白2和蛋白质二硫键异构酶（PDI或葡萄糖调节蛋白（GRP））。除了MDH外，已知PMT-2、肌联蛋白帽蛋白和蛋白质二硫键异构酶（PDI）与代谢有关。然而，目前尚不清楚给予Lyprinol是否会降低体内代谢葡萄糖水平并减轻炎症和关节炎症状。需要进一步的实验来关联柠檬酸中间体和葡萄糖水平与喂食Lyprinol的AIA大鼠的炎症和疼痛严重程度。
