Aguirre Enara, Ayllón Tania, Sainz Angel, Amusategui Inmaculada, Villaescusa Alejandra, Rodríguez-Franco Fernando, Tesouro Miguel A
Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.
Vet J. 2009 Nov;182(2):301-5. doi: 10.1016/j.tvjl.2008.07.013. Epub 2008 Aug 28.
An indirect fluorescent antibody (IFA) test is usually performed to detect antibodies in dogs naturally infected by Ehrlichia canis. In this work, results obtained using three different E. canis strains as antigen (a commercial antigen, the E. canis Oklahoma strain and the E. canis Madrid strain) were compared. One hundred and forty-nine serum samples obtained from dogs living in the centre of Spain were analysed. When qualitative results were evaluated, identical results were detected in 87.2% of samples for the three antigens tested. When comparing antibody titre results, differences between the Madrid strain and the commercial antigen, and between the Madrid and Oklahoma strains were statistically significant (P<0.0001). No differences were found when comparing the Oklahoma strain with the commercial antigen (P=0.562). Subtle intra-laboratory variations shown in this study suggest a higher sensitivity of the IFA test when an autochthonous strain is used as antigen.
通常采用间接荧光抗体（IFA）试验来检测自然感染犬埃立克体的犬体内的抗体。在这项研究中，比较了使用三种不同的犬埃立克体菌株作为抗原（一种商业抗原、犬埃立克体俄克拉荷马菌株和犬埃立克体马德里菌株）所获得的结果。对从西班牙中部地区的犬只采集的149份血清样本进行了分析。在评估定性结果时，所检测的三种抗原在87.2%的样本中检测到相同结果。在比较抗体滴度结果时，马德里菌株与商业抗原之间以及马德里菌株与俄克拉荷马菌株之间的差异具有统计学意义（P<0.0001）。将俄克拉荷马菌株与商业抗原进行比较时未发现差异（P=0.562）。本研究显示的实验室内部细微差异表明，当使用本地菌株作为抗原时，IFA试验具有更高的敏感性。
