使用两种不同诊断测试对墨西哥尤卡坦半岛流浪狗中的犬埃立克体进行血清学调查。

Serological survey of Ehrlichia canis in stray dogs from Yucatan, Mexico, using two different diagnostic tests.

作者信息

Jiménez-Coello M, Pérez-Osorio C, Vado-Solís I, Rodríguez-Buenfil J C, Ortega-Pacheco A

机构信息

Laboratorio de Biología Celular, Biomedicina de Enf Infecciosas y Parasitarias, Centro de Investigaciones Regionales Hideyo Noguchi, Universidad Autónoma de Yucatán, Mérida, Yucatán, México.

出版信息

Vector Borne Zoonotic Dis. 2009 Apr;9(2):209-12. doi: 10.1089/vbz.2008.0039.

DOI:10.1089/vbz.2008.0039

PMID:18945189

Abstract

Canine ehrlichiosis is a disease produced by the rickettsial organism Ehrlichia canis. Reported prevalence may vary greatly depending on the test and sampling method used. For the serological detection of antibodies against E. canis, the indirect immunofluorescence antibody test (IFA) is considered the gold standard. However, other available serological techniques such as the indirect immunoperoxidase technique (IPT) have not yet been tested and may be efficient in detecting specific antibodies. Prevalence found (8.7% and 8.1% for IFA and IPT, respectively) was lower than previously reported in the studied area despite the well-established technique used for the determination of antigens. A kappa value of 0.958 (95% CI 0.9-1.0) was found with a sensitivity and specificity for IPT of 92.59% (95% CI 80.8-99.9) and 99.9% (95% CI 99.8 -100), respectively. The positive predictive value was 99.9% and the negative predictive value was 99.29%. The IPT technique can be used safely for serological determination of E. canis antibodies.

摘要

犬埃立克体病是由立克次氏体病原体犬埃立克体引起的一种疾病。报告的患病率可能因所使用的检测和采样方法而有很大差异。对于犬埃立克体抗体的血清学检测，间接免疫荧光抗体试验（IFA）被认为是金标准。然而，其他可用的血清学技术，如间接免疫过氧化物酶技术（IPT）尚未经过测试，可能在检测特异性抗体方面很有效。尽管使用了成熟的抗原测定技术，但发现的患病率（IFA和IPT分别为8.7%和8.1%）低于该研究区域先前报告的患病率。IPT的kappa值为0.958（95%可信区间0.9 - 1.0），敏感性和特异性分别为92.59%（95%可信区间80.8 - 99.9）和99.9%（95%可信区间99.8 - 100）。阳性预测值为99.9%，阴性预测值为99.29%。IPT技术可安全用于犬埃立克体抗体的血清学测定。